Substituted heterocycles as antiviral agents

ABSTRACT

which inhibit the protein(s) encoded by hepatitis B virus (HBV) or interfere with the function of the HBV life cycle of the hepatitis B virus and are also useful as antiviral agents. The present invention further relates to pharmaceutical compositions comprising the aforementioned compounds for administration to a subject suffering from HBV infection. The invention also relates to methods of treating an HBV infection in a subject by administering a pharmaceutical composition comprising the compounds of the present invention.

RELATED APPLICATION

This application claims the benefit of U.S. Provisional Application No. 62/872,933, filed on Jul. 11, 2019. The entire teachings of the above application are incorporated herein by reference.

TECHNICAL FIELD

The present invention relates generally to compounds and pharmaceutical compositions useful as hepatitis virus replication inhibitors. Specifically, the present invention relates to tetracyclic pyridone compounds that are useful in treating viral infections such as hepatitis B virus (HBV). The invention provides novel tetracyclic pyridone compounds as disclosed herein, pharmaceutical compositions containing such compounds, and methods of using these compounds and compositions in the treatment and prevention of HBV infections.

BACKGROUND OF THE INVENTION

Over 240 million people throughout the world are chronically infected with hepatitis B virus (HBV). Out of this patient population, at least 2 million reside in the United States. For those that are chronically infected, many will develop complications of liver disease from cirrhosis or hepatocellular carcinoma (HCC).

HBV is a member of the Hepadnavirus family, and it is able to replicate through the reverse transcription of an RNA intermediate. The 3.2-kb HBV genome exists in a circular, partially doublestranded DNA conformation (rcDNA) that has four overlapping open reading frames (ORF). These encode for the core, polymerase, envelope, and X proteins of the virus. rcDNA must be converted into covalently closed circular DNA (cccDNA) in cells prior to the transcription of viral RNAs. As rcDNA is transcriptionally inert, cccDNA is the only template for HBV transcription, and its existence is required for infection.

The HBV viral envelope contains a mixture of surface antigen proteins (HBsAg). The HBsAg coat contains three proteins that share a common region that includes the smallest of the three proteins (SHBsAg). The other two proteins, Medium HBsAg (MHBsAg) and Large HBsAg (LHBsAg), both contain a segment of SHBsAg with additional polypeptide segments. SHBsAg, MHBsAg, and LHBsAg can also assemble into a non-infectious subviral particle known as the 22-nm particle that contains the same proteins found around infectious viral particles. As the 22-nm particles contain the same antigenic surface proteins that exist around the infectious HBV virion, they can be used as a vaccine to produce neutralizing antibodies.

In chronically infected patients, the non-infectious 22-nm particles are found in much greater abundance than the infectious virions. As a result, the 22-nm particles are thought to be able to protect the infectious virions from the infected host's immune response. Not only can they serve as infectious decoys, but they also suppress normal functioning of immune cells thereby impairing the host's immune response to HBV. Therefore, reducing the level of subviral particles is a feasible therapeutic approach to treating HBV infections. (Refer to WO2015/13990).

In the clinical setting, a diagnostic marker of chronic HBV infection is high serum levels of HBsAg. In recent years, data have suggested that sustained virologic response (SVR) corresponds with HBsAg decline during early treatment, while sustained exposure to HBsAg and other viral antigens might lead to inept immunogenicity. Patients that display higher decreases in serum HBsAg reached a considerably higher SVR following treatment.

Current treatment options for chronically infected HBV patients are limited in number and scope. They include interferon therapy and nucleoside-based inhibitors of HBV DNA polymerase, namely entecavir and tenofovir. The current standard of care is dedicated to reducing the level of viremia and allowance of liver dysfunction, but is associated with negative side-effects and increase persistence of drug-resistant HBV mutants. A significant shortcoming of current therapies is that they are unable to eliminate hepatic resevoirs of cccDNA, prevent transcription of HBsAg from cccDNA, or limit the secretion of HBsAg into serum that will ultimately stifle the immune response. Although compounds have been reported to reduce serum HBsAg levels, they have not been approved as HBV therapies. (Refer to WO2015/113990, WO2015/173164, WO2016/023877, WO2016/071215, WO2016/128335, WO 2017/140821, WO2019097479, WO2019166951, WO2019123285, WO2018198079, WO2018073753, WO2018047109, WO2019110352, WO2019129681, WO2018087345, WO2018083136, WO2018083106, WO2018083081, WO2017216391, WO2018001952, WO2018001944, WO2016107832, WO2016177655, WO2017017042, WO2017017043. WO2017013046, WO2016128335, WO2016071215, WO2015173164, WO2015113990, WO2018219356, WO2018130152, WO2018154466, WO2019069293, WO2017061466, WO2018181883, WO2018161960, WO2017205115, WO2018144605, WO2018085619, WO2018019297, and WO2018022282).

More effective therapies for chronic HBV infections are needed due to this high unmet clinical need. This invention describes the methods to prepare and methods for use of compounds that are believed to suppress the secretion of subviral particles containing HBsAg. Compounds of this type might be used to treat HBV infections and decrease occurrence of liver disease complications such as cirrhosis or HCC.

There is a need in the art for novel therapeutic agents that treat, ameliorate or prevent HBV infection. Administration of these therapeutic agents to an HBV infected patient, either as monotherapy or in combination with other HBV treatments or ancillary treatments, will lead to significantly improved prognosis, diminished progression of the disease, and enhanced seroconversion rates.

SUMMARY OF THE INVENTION

The present invention relates to novel antiviral compounds, pharmaceutical compositions comprising such compounds, as well as methods to treat or prevent viral (particularly HBV) infection in a subject in need of such therapy with said compounds. Compounds of the present invention inhibit the protein(s) encoded by hepatitis B virus (HBV) or interfere with the life cycle of HBV and are also useful as antiviral agents. In addition, the present invention includes the process for the preparation of the said compounds.

The present invention provides compounds represented by Formula (I),

and pharmaceutically acceptable salts, esters and prodrugs thereof, wherein:

R₁ is hydrogen, halo, or optionally substituted C₁-C₆ alkyl;

R₂ is hydrogen, halo, CN, optionally substituted C₁-C₆ alkyl, or optionally substituted C₁-C₆ alkoxy;

R₃ is hydrogen, halo, CN, optionally substituted C₁-C₆ alkyl, optionally substituted C₂-C₆ alkenyl; optionally substituted C₁-C₆ alkoxy, optionally substituted C₃-C₁₂ cycloalkyl, optionally substituted C₃-C₁₂ cycloalkenyl, optionally substituted 3- to 12-membered heterocyclic, optionally substituted aryl, or optionally substituted heteroaryl;

A is optionally substituted 3- to 12-membered heterocyclic, optionally substituted aryl, or optionally substituted heteroaryl; preferably A is optionally substituted 5- to 7-membered unsaturated heterocyclic, or optionally substituted 5- to 6-membered heteroaryl.

R₄ is hydrogen, halo, optionally substituted C₁-C₆ alkyl, or optionally substituted C₁-C₆ alkoxy;

R₅ is hydrogen, halo, optionally substituted C₁-C₆ alkyl, or optionally substituted C₁-C₆ alkoxy;

R₆ is hydrogen or optionally substituted C₁-C₆ alkyl;

R₇ is hydrogen, halo, optionally substituted C₁-C₆ alkyl, or optionally substituted C₁-C₆ alkoxy;

R₈ is hydrogen or optionally substituted methyl;

W is —COOR₁₁, —C(O)NHSO₂R₁₂, —C(O)NHSO₂N(R₁₂R₁₃), 5-tetrazolyl, or 1,2,4-oxadiazol-3-yl-5(4H)-one;

R₁₁ is hydrogen or optionally substituted C₁-C₆ alkyl; preferably the C₁-C₆ alkyl is optionally substituted with one or two groups independently selected from halo, —OR₁₂, oxo, CN, —N(R₁₂)(R₁₃), COOR₁₂, and CON(R₂)(R₁₃), and

R₁₂ and R₁₃ are each independently selected from hydrogen and optionally substituted C₁-C₆ alkyl;

Alternatively, R₆ is taken together with R₅ and the carbon atoms to which they are attached to form a C₃-C₇ cycloalkyl ring or a 3- to 7-membered heterocyclic ring; or R₆ is taken together with R₅ and the carbon atoms to which they are attached to form a C₄-C₇ cycloalkenyl ring; wherein the cycloalkyl, cycloalkenyl or heterocyclic ring is optionally substituted with up to three groups selected from R₁₂, —OR₁₂, —N(R₁₂)(R₁₃), halo, CN, COOR₁₂, CON(R₂)(R₁₃), and oxo; or R₇ is taken together with R₆ and the carbon atom to which they are attached to form a C₃-C₇ cycloalkyl ring or a 3- to 7-membered heterocyclic ring; wherein the cycloalkyl or heterocyclic ring is optionally substituted with up to three groups selected from R₁₂, —OR₁₂, —N(R₁₂)(R₃)₂, halo, CN, COOR₁₂, CON(R₂)(R₃)₂, and oxo.

Each preferred group stated above can be taken in combination with one, any or all other preferred groups.

DETAILED DESCRIPTION OF THE INVENTION

In one embodiment of the present invention is a compound of Formula (I) as described above, or a pharmaceutically acceptable salt thereof.

In certain embodiments of the compounds of Formula (I), R₁ is hydrogen, Cl, F, —CH₃ or —CF₃.

In certain embodiments of the compounds of Formula (I), R₂ is hydrogen, Cl or F.

In certain embodiments of the compounds of Formula (I), A is optionally substituted 5- to 7-membered unsaturated heterocyclic, or optionally substituted 5- to 6-membered heteroaryl.

In certain embodiments of the compounds of Formula (I), R₄ is hydrogen, and R₅ is hydrogen.

In certain embodiments of the compounds of Formula (I), R₆ is hydrogen, or optionally substituted C₁-C₆ alkyl. In certain embodiments, R₆ is t-butyl.

In certain embodiments of the compounds of Formula (I), R₇ is hydrogen, or optionally substituted C₁-C₆ alkyl.

In certain embodiments of the compounds of Formula (I), R₈ is hydrogen, or methyl optionally substituted with one or more halo, preferably fluoro.

In certain embodiments of the compounds of Formula (I), W is —COOH or —C(O)NHSO₂N(R₁₂)(R₁₃), wherein R₁₂ and R₁₃ are as previously defined.

In certain embodiments of the compounds of Formula (I), R₃ is hydrogen, halo, optionally substituted 3- to 12-membered heterocyclic, optionally substituted aryl, or optionally substituted heteroaryl. Preferably, R₃ is optionally substituted phenyl, optionally substituted 5- to 6-membered heterocyclic, or optionally substituted 5- to 6-membered heteroaryl.

In certain embodiments of the compounds of Formula (I), R₃ is selected from one of the following by removal of a hydrogen atom:

wherein each of these groups is optionally substituted with one to four groups selected from halo, CN, —OR₁₁, —N(R₁₂R₁₃), optionally substituted C₁-C₆ alkyl, and optionally substituted 3- to 7-membered heterocyclic. In certain embodiments, each of these groups is optionally substituted with one to four, preferably one or two, groups selected from fluoro, chloro, methyl, methoxy, trifluoromethyl and difluoromethyl.

In certain embodiments of the compounds of Formula (I), R₃ is selected from the groups set forth below:

wherein each of these groups is optionally substituted.

In certain embodiments of the compounds of Formula (I), A is selected from the groups set forth below:

wherein the two indicated valences are the points of attachment to either position of the phenyl ring in Formula (I) which is fused with the A ring.

In another embodiment, the compound of Formula (I) is represented by Formula (II), or a pharmaceutically acceptable salt thereof:

wherein R₁, R₂, R₃, A, R₄, R₅, R₆, R₇, R₈, and W are as previously defined.

In certain embodiments, the compounds of the invention have the absolute stereochemistry illustrated in Formula (II). In certain embodiments, R₆ and R₇ are taken together with the carbon atom to which they are attached to form a spiro ring. In certain embodiments, R₅ and R₆ are taken together with the carbon atoms to which they are attached to form a cis-fused ring. In certain embodiments, R₄ and R₇ are both hydrogen.

In another embodiment, the compound of Formula (I) is represented by Formula (IIIa) or Formula (IIIb), or a pharmaceutically acceptable salt thereof:

wherein one V is —O—, —C(O)—, —S—, —S(O)₂—, —NR₂₂— or —C(R₂₁)₂—, and the other Vs are independently —O—, —NR₂₂— or —C(R₂₁)₂—; each R₂₁ is independently hydrogen, halogen, optionally substituted —C₁-C₆ alkyl, optionally substituted —C₂-C₆ alkenyl, optionally substituted —C₂-C₆ alkynyl, optionally substituted C₁-C₆ alkoxy; optionally substituted —C₃-C₇ cycloalkyl, optionally substituted 3- to 7-membered heterocyclic, optionally substituted aryl or optionally substituted heteroaryl; each R₂₂ is independently hydrogen, optionally substituted —C₁-C₆ alkyl, optionally substituted —C₂-C₆ alkenyl, optionally substituted —C₂-C₆ alkynyl, optionally substituted C₁-C₆ alkoxy; optionally substituted —C₃-C₇ cycloalkyl, optionally substituted 3- to 7-membered heterocyclic, optionally substituted aryl or optionally substituted heteroaryl; n is 0, 1, 2 or 3; R₁, R₂, A, R₃, R₆, R₈ and W are as previously defined. In certain embodiments, each of two adjacent Vs is —C(R₂₁)₂—. In another embodiment, two adjacent Vs are taken together to form —C(R₂₁)═C(R₂₁)—.

In another embodiment, the compound of Formula (I) is represented by Formula (IIIb-1) or (IIIb-2), or a pharmaceutically acceptable salt thereof:

wherein R₁, R₂, R₃, A, V, R₈, and W are as previously defined. In certain embodiments, each of two adjacent V_(S) is —C(R₂₁)₂—. In another embodiment, two adjacent V_(S) are together to form —C(R₂₁)═C(R₂₁)—, R₂₁ is previously defined.

In another embodiment, the compound of Formula (I) is represented by Formula (IIIb-3) or (IIIb-4), or a pharmaceutically acceptable salt thereof:

wherein R₁, R₂, R₃, A, V, R₂₁, R₈, and W are as previously defined. In certain embodiments, each V is —C(R₂₁)₂—. In certain embodiments, two adjacent Vs together form —C(R₂)═C(R₂₁)—. In certain embodiments, each R₂₁ is independently hydrogen or methyl. In certain embodiments, each R₂₁ is methyl.

In another embodiment, the compound of Formula (I) is represented by Formulae (IIIb-5)˜(IIIb-12), or a pharmaceutically acceptable salt thereof:

wherein V′ is —NR₂₂— or —C(R₂₁)₂—; V″ is O, —NR₂₂—, or —C(R₂₁)₂—; R₂₁ and R₂₂ are previously defined, and each R₂₁ or R₂₂ can be same or different.

In certain embodiments, the present invention relates to compounds of Formulae (IIIb-1)˜(IIIb-12), or pharmaceutically acceptable salts thereof, wherein A is selected from the groups set forth below:

wherein the two indicated valences are the points of attachment to either position of the phenyl ring in Formula (I) which is fused with the A ring.

In another embodiment, the compound of Formula (I) is represented by Formula (IV), or a pharmaceutically acceptable salt thereof:

wherein R₁, R₃, A, R₆, and W are as previously defined.

In another embodiment, the compound of Formula (I) is represented by Formula (V), or a pharmaceutically acceptable salt thereof:

wherein one U is —O—, —S—, or —NR₂₂—, and the other Us are independently N, —NR₂₂—, or —C(R₂₁)₂—; wherein at least one pair of adjacent Us are taken together to form —C(R₂₁)═C(R₂₁)—, —C(R₂)═N—, or —N═N—; m is 0, 1, 2 or 3; preferably, m is 1 or 2; R₁, R₃, R₆, R₂₁, R₂₂, and W are as previously defined.

In another embodiment, the compound of Formula (V) is represented by Formulae (V-1)˜(V-4), or a pharmaceutically acceptable salt thereof:

wherein U¹ is O, S, or —NR₂₂— and T is N or —CR₂₁—; alternatively, U¹ is —C(R₂₁)₂— or —NR₂₂— and T is N. R₂₁ and R₂₂ are previously defined, and each R₂₁ or R₂₂ can be same or different.

In another embodiment, the compound of Formula (V) is represented by Formulae (V-5)˜(V-10), or a pharmaceutically acceptable salt thereof:

Wherein each U² is independently O, —C(R₂₁)₂—, or —NR₂₂—; each U is independently —CR₂₁—, or —N—. R₁, R₃, R₆, R₂₁, R₂₂, and W are previously defined, and each R₂₁ or R₂₂ can be same or different.

In another embodiment, the compound of Formula (I) is represented by Formula (VI-1) or Formula (VI-2), or a pharmaceutically acceptable salt thereof:

wherein R₃, A, R₆, and W are as previously defined.

In certain embodiments, the present invention relates to compounds of Formula (VI-1) or Formula (VI-2), or pharmaceutically acceptable salts thereof, wherein A is selected from the groups set forth below:

wherein the two indicated valences are the points of attachment to either position of the phenyl ring in Formula (I) which is fused with the A ring.

In another embodiment, the compound of Formula (I) is represented by Formulae (VII-1)˜(VII-4), or a pharmaceutically acceptable salt thereof:

wherein R₃ and R₆ are as previously defined.

In another embodiment, the compound of Formula (I) is represented by Formulae (VIII-1)˜(VIII-4), or a pharmaceutically acceptable salt thereof.

wherein R₁, R₃ and R₆ are as previously defined.

In certain embodiments, the present invention relates to compounds of Formulae (VII-1)˜(VII-4), or Formulae (VIII-1)˜(VIII-4), or pharmaceutically acceptable salts thereof, wherein R₃ is selected from the groups set forth below:

wherein each of these groups is optionally substituted.

In another embodiment, the compound of Formula (I) is represented by Formulae (IX-1)˜(IX-4), or a pharmaceutically acceptable salt thereof:

wherein one X is O or CH₂, the other X is CH₂; and R₃ is as previously defined.

It will be appreciated that the description of the present invention herein should be construed in congruity with the laws and principles of chemical bonding. In some instances, it may be necessary to remove a hydrogen atom in order to accommodate a substituent at any given location.

It will be yet appreciated that the compounds of the present invention may contain one or more asymmetric carbon atoms and may exist in racemic, diastereoisomeric, and optically active forms. It will still be appreciated that certain compounds of the present invention may exist in different tautomeric forms. All tautomers are contemplated to be within the scope of the present invention.

In one embodiment, the compounds described herein are suitable for monotherapy and are effective against natural or native HBV strains and against HBV strains resistant to currently known drugs. In another embodiment, the compounds described herein are suitable for use in combination therapy.

In another embodiment, the compounds of the invention can be used in methods of modulating (e.g., inhibit, disrupt or accelerate) the activity of HBV cccDNA. In yet another embodiment, the compounds of the invention can be used in methods of diminishing or preventing the formation of HBV cccDNA. In another embodiment, the additional therapeutic agent is selected from core inhibitor, which includes GLS4, GLS4JHS, JNJ-379, ABI-H731, ABI-H2158, AB-423, AB-506, WX-066, and QL-0A6A; immune modulator or immune stimulator therapies, which includes T-cell response activator AIC649 and biological agents belonging to the interferon class, such as interferon alpha 2a or 2b or modified interferons such as pegylated interferon, alpha 2a, alpha 2b, lamda; or STING (stimulator of interferon genes) modulator; or TLR modulators such as TLR-7 agonists, TLR-8 agonists or TLR-9 agonists; or therapeutic vaccines to stimulate an HBV-specific immune response such as virus-like particles composed of HBcAg and HBsAg, immune complexes of HBsAg and HBsAb, or recombinant proteins comprising HBx, HBsAg and HBcAg in the context of a yeast vector; or immunity activator such as SB-9200 of certain cellular viral RNA sensors such as RIG-I, NOD2, and MDA5 protein, or RNA interence (RNAi) or small interfering RNA (siRNA) such as ARC-520, ARC-521, ARB-1467, and ALN-HBV RNAi, or antiviral agents that block viral entry or maturation or target the HBV polymerase such as nucleoside or nucleotide or non-nucleos(t)ide polymerase inhibitors, and agents of distinct or unknown mechanism including agents that disrupt the function of other essential viral protein(s) or host proteins required for HBV replication or persistence such as REP 2139, RG7834, and AB-452. In an embodiment of the combination therapy, the reverse transcriptase inhibitor is at least one of Zidovudine, Didanosine, Zalcitabine, ddA, Stavudine, Lamivudine, Aba-cavir, Emtricitabine, Entecavir, Apricitabine, Atevirapine, ribavirin, acyclovir, famciclovir, valacyclovir, ganciclovir, valganciclovir, Tenofovir, Adefovir, PMPA, cidofovir, Efavirenz, Nevirapine, Delavirdine, or Etravirine.

In another embodiment of the combination therapy, the TLR-7 agonist is selected from the group consisting of SM360320 (9-benzyl-8-hydroxy-2-(2-methoxy-ethoxy)ad-enine), AZD 8848 (methyl [3-({[3-(6-amino-2-butoxy-8-oxo-7,8-dihydro-9H-purin-9-yl)propyl][3-(4-morpholinyl) propyl]amino Imethyl)phenyl]acetate), GS-9620 (4-Amino-2-butoxy-8-[3-(1-pyrrolidinylmethyl)benzyl]-7,8-dihydro-6(5H)-pteridinone), AL-034 (TQ-A3334), and RO6864018.

In another embodiment of the combination therapy, the TLR-8 agonist is GS-9688.

In an embodiment of these combination therapies, the compound and the additional therapeutic agent are co-formulated. In another embodiment, the compound and the additional therapeutic agent are co-administered.

In another embodiment of the combination therapy, administering the compound of the invention allows for administering of the additional therapeutic agent at a lower dose or frequency as compared to the administering of the at least one additional therapeutic agent alone that is required to achieve similar results in prophylactically treating an HBV infection in an individual in need thereof.

In another embodiment of the combination therapy, before administering the therapeutically effective amount of the compound of the invention, the individual is known to be refractory to a compound selected from the group consisting of a HBV polymerase inhibitor, interferon, viral entry inhibitor, viral maturation inhibitor, distinct capsid assembly modulator, antiviral compounds of distinct or unknown mechanism, and combination thereof.

In still another embodiment of the method, administering the compound of the invention reduces viral load in the individual to a greater extent compared to the administering of a compound selected from the group consisting of a HBV polymerase inhibitor, interferon, viral entry inhibitor, viral maturation inhibitor, distinct capsid assembly modulator, antiviral compounds of distinct or unknown mechanism, and combination thereof.

In another embodiment, administering of the compound of the invention causes a lower incidence of viral mutation and/or viral resistance than the administering of a compound selected from the group consisting of a HBV polymerase inhibitor, interferon, viral entry inhibitor, viral maturation inhibitor, distinct capsid assembly modulator, antiviral compounds of distinct or unknown mechanism, and combination thereof.

It should be understood that the compounds encompassed by the present invention are those that are suitably stable for use as pharmaceutical agent.

Definitions

Listed below are definitions of various terms used to describe this invention. These definitions apply to the terms as they are used throughout this specification and claims, unless otherwise limited in specific instances, either individually or as part of a larger group.

The term “aryl,” as used herein, refers to a mono- or polycyclic carbocyclic ring system comprising at least one aromatic ring, including, but not limited to, phenyl, naphthyl, tetrahydronaphthyl, indanyl, and indenyl. A polycyclic aryl is a polycyclic ring system that comprises at least one aromatic ring. Polycyclic aryls can comprise fused rings, covalently attached rings or a combination thereof.

The term “heteroaryl,” as used herein, refers to a mono- or polycyclic aromatic radical having one or more ring atom selected from S, O and N; and the remaining ring atoms are carbon, wherein any N or S contained within the ring may be optionally oxidized. Heteroaryl includes, but is not limited to, pyridinyl, pyrazinyl, pyrimidinyl, pyrrolyl, pyrazolyl, imidazolyl, thiazolyl, oxazolyl, isooxazolyl, thiadiazolyl, oxadiazolyl, thiophenyl, furanyl, quinolinyl, isoquinolinyl, benzimidazolyl, benzoxazolyl, quinoxalinyl. A polycyclic heteroaryl can comprise fused rings, covalently attached rings or a combination thereof.

In accordance with the invention, aromatic groups can be substituted or unsubstituted.

The term “alkyl” as used herein, refers to saturated, straight- or branched-chain hydrocarbon radicals. “C₁-C₄ alkyl,” “C₁-C₆ alkyl,” “C₁-C₈ alkyl,” “C₁-C₁₂ alkyl,” “C₂-C₄ alkyl,” or “C₃-C₆ alkyl,” refer to alkyl groups containing from one to four, one to six, one to eight, one to twelve, 2 to 4 and 3 to 6 carbon atoms respectively. Examples of C₁-C₈ alkyl radicals include, but are not limited to, methyl, ethyl, propyl, isopropyl, n-butyl, tert-butyl, neopentyl, n-hexyl, heptyl and octyl radicals.

The term “alkenyl” as used herein, refers to straight- or branched-chain hydrocarbon radicals having at least one carbon-carbon double bond by the removal of a single hydrogen atom. “C₂-C₈ alkenyl,” “C₂-C₁₂ alkenyl,” “C₂-C₄ alkenyl,” “C₃-C₄ alkenyl,” or “C₃-C₆ alkenyl,” refer to alkenyl groups containing from two to eight, two to twelve, two to four, three to four or three to six carbon atoms respectively. Alkenyl groups include, but are not limited to, for example, ethenyl, propenyl, butenyl, 1-methyl-2-buten-1-yl, heptenyl, octenyl, and the like.

The term “alkynyl” as used herein, refers to straight- or branched-chain hydrocarbon radicals having at least one carbon-carbon double bond by the removal of a single hydrogen atom. “C₂-C₈ alkynyl,” “C₂-C₁₂ alkynyl,” “C₂-C₄ alkynyl,” “C₃-C₄ alkynyl,” or “C₃-C₆ alkynyl,” refer to alkynyl groups containing from two to eight, two to twelve, two to four, three to four or three to six carbon atoms respectively. Representative alkynyl groups include, but are not limited to, for example, ethynyl, 1-propynyl, 1-butynyl, heptynyl, octynyl, and the like.

The term “cycloalkyl”, as used herein, refers to a monocyclic or polycyclic saturated carbocyclic ring or a bi- or tri-cyclic group fused, bridged or spiro system, and the carbon atoms may be optionally oxo-substituted or optionally substituted with exocyclic olefinic double bond. Preferred cycloalkyl groups include C₃-C₁₂ cycloalkyl, C₃-C₆ cycloalkyl, C₃-C₈ cycloalkyl and C₄-C₇ cycloalkyl. Examples of C₃-C₁₂ cycloalkyl include, but not limited to, cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cyclopentyl, cyclooctyl, 4-methylene-cyclohexyl, bicyclo[2.2.1]heptyl, bicyclo[3.1.0]hexyl, spiro[2.5]octyl, 3-methylenebicyclo[3.2.1]octyl, spiro[4.4]nonanyl, and the like.

The term “cycloalkenyl”, as used herein, refers to monocyclic or polycyclic carbocyclic ring or a bi- or tri-cyclic group fused, bridged or spiro system having at least one carbon-carbon double bond and the carbon atoms may be optionally oxo-substituted or optionally substituted with exocyclic olefinic double bond. Preferred cycloalkenyl groups include C₃-C₁₂ cycloalkenyl, C₃-C₈ cycloalkenyl or C₅-C₇ cycloalkenyl groups. Examples of C₃-C₁₂ cycloalkenyl include, but not limited to, cyclopropenyl, cyclobutenyl, cyclopentenyl, cyclohexenyl, cycloheptenyl, cyclooctenyl, bicyclo[2.2.1]hept-2-enyl, bicyclo[3.1.0]hex-2-enyl, spiro[2.5]oct-4-enyl, spiro[4.4]non-1-enyl, bicyclo[4.2.1]non-3-en-9-yl, and the like.

As used herein, the term “arylalkyl” means a functional group wherein an alkylene chain is attached to an aryl group, e.g., —CH₂CH₂-phenyl. The term “substituted arylalkyl” means an arylalkyl functional group in which the aryl group is substituted. Similarly, the term “heteroarylalkyl” means a functional group wherein an alkylene chain is attached to a heteroaryl group. The term “substituted heteroarylalkyl” means a heteroarylalkyl functional group in which the heteroaryl group is substituted.

As used herein, the term “alkoxy” employed alone or in combination with other terms means, unless otherwise stated, an alkyl group having the designated number of carbon atoms connected to the rest of the molecule via an oxygen atom, such as, for example, methoxy, ethoxy, 1-propoxy, 2-propoxy (isopropoxy) and the higher homologs and isomers. Preferred alkoxy are (C₁-C₃) alkoxy.

It is understood that any alkyl, alkenyl, alkynyl, cycloalkyl, heterocyclic and cycloalkenyl moiety described herein can also be an aliphatic group or an alicyclic group.

An “aliphatic” group is a non-aromatic moiety comprised of any combination of carbon atoms, hydrogen atoms, halogen atoms, oxygen, nitrogen or other atoms, and optionally contains one or more units of unsaturation, e.g., double and/or triple bonds. Examples of aliphatic groups are functional groups, such as alkyl, alkenyl, alkynyl, O, OH, NH, NH₂, C(O), S(O)₂, C(O)O, C(O)NH, OC(O)O, OC(O)NH, OC(O)NH₂, S(O)₂NH, S(O)₂NH₂, NHC(O)NH₂, NHC(O)C(O)NH, NHS(O)₂NH, NHS(O)₂NH₂, C(O)NHS(O)₂, C(O)NHS(O)₂NH or C(O)NHS(O)₂NH₂, and the like, groups comprising one or more functional groups, non-aromatic hydrocarbons (optionally substituted), and groups wherein one or more carbons of a non-aromatic hydrocarbon (optionally substituted) is replaced by a functional group. Carbon atoms of an aliphatic group can be optionally oxo-substituted. An aliphatic group may be straight chained, branched, cyclic, or a combination thereof and preferably contains between about 1 and about 24 carbon atoms, more typically between about 1 and about 12 carbon atoms. In addition to aliphatic hydrocarbon groups, as used herein, aliphatic groups expressly include, for example, alkoxyalkyls, polyalkoxyalkyls, such as polyalkylene glycols, polyamines, and polyimines, for example. Aliphatic groups may be optionally substituted.

The terms “heterocyclic” or “heterocycloalkyl” can be used interchangeably and referred to a non-aromatic ring or a bi- or tri-cyclic group fused, bridged or spiro system, where (i) each ring system contains at least one heteroatom independently selected from oxygen, sulfur and nitrogen, (ii) each ring system can be saturated or unsaturated, (iii) the nitrogen and sulfur heteroatoms may optionally be oxidized, (iv) the nitrogen heteroatom may optionally be quaternized, (v) any of the above rings may be fused to an aromatic ring, and (vi) the remaining ring atoms are carbon atoms which may be optionally oxo-substituted or optionally substituted with exocyclic olefinic double bond. Representative heterocycloalkyl groups include, but are not limited to, 1,3-dioxolane, pyrrolidinyl, pyrazolinyl, pyrazolidinyl, imidazolinyl, imidazolidinyl, piperidinyl, piperazinyl, oxazolidinyl, isoxazolidinyl, morpholinyl, thiazolidinyl, isothiazolidinyl, quinoxalinyl, pyridazinonyl, 2-azabicyclo[2.2.1]-heptyl, 8-azabicyclo[3.2.1]octyl, 5-azaspiro[2.5]octyl, 1-oxa-7-azaspiro[4.4]nonanyl, 7-oxooxepan-4-yl, and tetrahydrofuryl. Such heterocyclic groups may be further substituted. Heteroaryl or heterocyclic groups can be C-attached or N-attached (where possible).

It is understood that any alkyl, alkenyl, alkynyl, alicyclic, cycloalkyl, cycloalkenyl, aryl, heteroaryl, heterocyclic, aliphatic moiety or the like, described herein can also be a divalent or multivalent group when used as a linkage to connect two or more groups or substituents, which can be at the same or different atom(s). One of skill in the art can readily determine the valence of any such group from the context in which it occurs. For example, an alkyl group which has an open valence on two different carbon atoms can also be referred to as an alkylene group.

The term “substituted” refers to substitution by independent replacement of one, two, or three or more of the hydrogen atoms with substituents including, but not limited to, —F, —Cl, —Br, —I, —OH, C₁-C₁₂-alkyl; C₂-C₁₂-alkenyl, C₂-C₁₂-alkynyl, —C₃-C₂-cycloalkyl, protected hydroxy, —NO₂, —N₃, —CN, —NH₂, protected amino, oxo, thioxo, —NH—C₁-C₂-alkyl, —NH—C₂-C₈-alkenyl, —NH—C₂-C₈-alkynyl, —NH—C₃-C₂-cycloalkyl, —NH-aryl, —NH-heteroaryl, —NH-heterocycloalkyl, -dialkylamino, -diarylamino, -diheteroarylamino, —O—C₁-C₁₂-alkyl, —O—C₂-C₈-alkenyl, —O—C₂-C₈-alkynyl, —O—C₃-C₁₂-cycloalkyl, —O-aryl, —O-heteroaryl, —O-heterocycloalkyl, —C(O)—C₁-C₁₂-alkyl, —C(O)—C₂-C₈-alkenyl, —C(O)—C₂-C₈-alkynyl, —C(O)—C₃-C₁₂-cycloalkyl, —C(O)-aryl, —C(O)— heteroaryl, —C(O)-heterocycloalkyl, —CONH₂, —CONH—C₁-C₁₂-alkyl, —CONH—C₂-C₈-alkenyl, —CONH—C₂-C₈-alkynyl, —CONH—C₃-C₁₂-cycloalkyl, —CONH-aryl, —CONH-heteroaryl, —CONH— heterocycloalkyl, —OCO₂—C₁-C₁₂-alkyl, —OCO₂—C₂-C₈-alkenyl, —OCO₂—C₂-C₈-alkynyl, —OCO₂—C₃-C₂-cycloalkyl, —OCO₂-aryl, —OCO₂-heteroaryl, —OCO₂-heterocycloalkyl, —CO₂—C₁-C₁₂ alkyl, —CO₂—C₂-C₈ alkenyl, —CO₂—C₂-C₈ alkynyl, CO₂—C₃-C₁₂-cycloalkyl, —CO₂-aryl, CO₂-heteroaryl, CO₂-heterocyloalkyl, —OCONH₂, —OCONH—C₁-C₁₂-alkyl, —OCONH—C₂-C₈-alkenyl, —OCONH—C₂-C₈-alkynyl, —OCONH—C₃-C₂-cycloalkyl, —OCONH-aryl, —OCONH-heteroaryl, —OCONH-heterocycloalkyl, —NHC(O)H, —NHC(O)—C₁-C₁₂-alkyl, —NHC(O)—C₂-C₈-alkenyl, —NHC(O)—C₂-C₈-alkynyl, —NHC(O)—C₃-C₂-cycloalkyl, —NHC(O)-aryl, —NHC(O)-heteroaryl, —NHC(O)-heterocyclo-alkyl, —NHCO₂—C₁-C₁₂-alkyl, —NHCO₂—C₂-C₈-alkenyl, —NHCO₂— C₂-C₈-alkynyl, —NHCO₂—C₃-C₁₂-cycloalkyl, —NHCO₂-aryl, —NHCO₂-heteroaryl, —NHCO₂-heterocycloalkyl, —NHC(O)NH₂, —NHC(O)NH—C₁-C₁₂-alkyl, —NHC(O)NH—C₂-C₈-alkenyl, —NHC(O)NH—C₂-C₈-alkynyl, —NHC(O)NH—C₃-C₁₂-cycloalkyl, —NHC(O)NH-aryl, —NHC(O)NH-heteroaryl, —NHC(O)NH-heterocycloalkyl, NHC(S)NH₂, —NHC(S)NH—C₁-C₁₂-alkyl, —NHC(S)NH—C₂-C₈-alkenyl, —NHC(S)NH—C₂-C₈-alkynyl, —NHC(S)NH—C₃-C₁₂-cycloalkyl, —NHC(S)NH-aryl, —NHC(S)NH-heteroaryl, —NHC(S)NH-heterocycloalkyl, —NHC(NH)NH₂, —NHC(NH)NH—C₁-C₁₂-alkyl, —NHC(NH)NH—C₂-C₈-alkenyl, —NHC(NH)NH—C₂-C₈-alkynyl, —NHC(NH)NH—C₃-C₁₂-cycloalkyl, —NHC(NH)NH-aryl, —NHC(NH)NH-heteroaryl, —NHC(NH)NH— heterocycloalkyl, —NHC(NH)—C₁-C₁₂-alkyl, —NHC(NH)—C₂-C₈-alkenyl, —NHC(NH)—C₂-C₈-alkynyl, —NHC(NH)—C₃-C₁₂-cycloalkyl, —NHC(NH)-aryl, —NHC(NH)-heteroaryl, —NHC(NH)— heterocycloalkyl, —C(NH)NH—C₁-C₁₂-alkyl, —C(NH)NH—C₂-C₈-alkenyl, —C(NH)NH—C₂-C₈-alkynyl, —C(NH)NH—C₃-C₁₂-cycloalkyl, —C(NH)NH-aryl, —C(NH)NH-heteroaryl, —C(NH)NH— heterocycloalkyl, —S(O)—C₁-C₁₂-alkyl, —S(O)—C₂-C₈-alkenyl, —S(O)—C₂-C₈-alkynyl, —S(O)—C₃-C₁₂-cycloalkyl, —S(O)-aryl, —S(O)-heteroaryl, —S(O)-heterocycloalkyl, —SO₂NH₂, —SO₂NH—C₁-C₁₂-alkyl, —SO₂NH—C₂-C₈-alkenyl, —SO₂NH— C₂-C₈-alkynyl, —SO₂NH—C₃-C₁₂-cycloalkyl, —SO₂NH-aryl, —SO₂NH-heteroaryl, —SO₂NH-heterocycloalkyl, —NHSO₂—C₁-C₁₂-alkyl, —NHSO₂—C₂-C₈-alkenyl, —NHSO₂—C₂-C₈-alkynyl, —NHSO₂—C₃-C₁₂-cycloalkyl, —NHSO₂-aryl, —NHSO₂-heteroaryl, —NHSO₂-heterocycloalkyl, —CH₂NH₂, —CH₂SO₂CH₃, -aryl, -arylalkyl, -heteroaryl, -heteroarylalkyl, -heterocycloalkyl, —C₃-C₁₂-cycloalkyl, polyalkoxyalkyl, polyalkoxy, -methoxymethoxy, -methoxyethoxy, —SH, —S—C₁-C₁₂-alkyl, —S—C₂-C₈-alkenyl, —S—C₂-C₈-alkynyl, —S—C₃-C₂-cycloalkyl, —S-aryl, —S-heteroaryl, —S-heterocycloalkyl, or methylthio-methyl. In certain embodiments, the substituents are independently selected from halo, preferably Cl and F; C₁-C₄-alkyl, preferably methyl and ethyl; halo-C₁-C₄-alkyl, such as fluoromethyl, difluoromethyl, and trifluoromethyl; C₂-C₄-alkenyl; halo-C₂-C₄-alkenyl; C₃-C₆-cycloalkyl, such as cyclopropyl; C₁-C₄-alkoxy, such as methoxy and ethoxy; halo-C₁-C₄-alkoxy, such as fluoromethoxy, difluoromethoxy, and trifluoromethoxy, —CN; —OH; NH; C₁-C₄-alkylamino; di(C₁-C₄-alkyl)amino; and NO₂. It is understood that the aryls, heteroaryls, alkyls, and the like can be further substituted. In some cases, each substituent in a substituted moiety is additionally optionally substituted when possible with one or more groups, each group being independently selected from C₁-C₄-alkyl; —CF₃, —OCH₃, —OCF₃, —F, —Cl, —Br, —I, —OH, —NO₂, —CN, and —NH₂. Preferably, a substituted alkyl group, such as a substituted methyl group, is substituted with one or more halogen atoms, more preferably one or more fluorine or chlorine atoms.

The term “halo” or halogen” alone or as part of another substituent, as used herein, refers to a fluorine, chlorine, bromine, or iodine atom.

The term “optionally substituted”, as used herein, means that the referenced group may be substituted or unsubstituted. In one embodiment, the referenced group is optionally substituted with zero substituents, i.e., the referenced group is unsubstituted. In another embodiment, the referenced group is optionally substituted with one or more additional group(s) individually and independently selected from groups described herein.

The term “hydrogen” includes hydrogen and deuterium. In addition, the recitation of an atom includes other isotopes of that atom so long as the resulting compound is pharmaceutically acceptable.

The term “hydroxy activating group,” as used herein, refers to a labile chemical moiety which is known in the art to activate a hydroxyl group so that it will depart during synthetic procedures such as in a substitution or an elimination reaction. Examples of hydroxyl activating group include, but not limited to, mesylate, tosylate, triflate, p-nitrobenzoate, phosphonate and the like.

The term “activated hydroxyl,” as used herein, refers to a hydroxy group activated with a hydroxyl activating group, as defined above, including mesylate, tosylate, triflate, p-nitrobenzoate, phosphonate groups, for example.

The term “hydroxy protecting group,” as used herein, refers to a labile chemical moiety which is known in the art to protect a hydroxyl group against undesired reactions during synthetic procedures. After said synthetic procedure(s) the hydroxy protecting group as described herein may be selectively removed. Hydroxy protecting groups as known in the art are described generally in T. H. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis, 3rd edition, John Wiley & Sons, New York (1999). Examples of hydroxyl protecting groups include benzyloxycarbonyl, 4-methoxybenzyloxycarbonyl, tert-butoxy-carbonyl, isopropoxycarbonyl, diphenylmethoxycarbonyl, 2,2,2-trichloroethoxycarbonyl, allyloxycarbonyl, acetyl, formyl, chloroacetyl, trifluoroacetyl, methoxyacetyl, phenoxyacetyl, benzoyl, methyl, t-butyl, 2,2,2-trichloroethyl, 2-trimethylsilyl ethyl, allyl, benzyl, triphenyl-methyl (trityl), methoxymethyl, methylthiomethyl, benzyloxymethyl, 2-(trimethylsilyl)-ethoxymethyl, methanesulfonyl, trimethylsilyl, triisopropylsilyl, and the like.

The term “protected hydroxy,” as used herein, refers to a hydroxy group protected with a hydroxy protecting group, as defined above, including benzoyl, acetyl, trimethylsilyl, triethylsilyl, methoxymethyl groups, for example.

The term “hydroxy prodrug group,” as used herein, refers to a promoiety group which is known in the art to change the physicochemical, and hence the biological properties of a parent drug in a transient manner by covering or masking the hydroxy group. After said synthetic procedure(s), the hydroxy prodrug group as described herein must be capable of reverting back to hydroxy group in vivo. Hydroxy prodrug groups as known in the art are described generally in Kenneth B. Sloan, Prodrugs, Topical and Ocular Drug Delivery, (Drugs and the Pharmaceutical Sciences; Volume 53), Marcel Dekker, Inc., New York (1992).

The term “amino protecting group,” as used herein, refers to a labile chemical moiety which is known in the art to protect an amino group against undesired reactions during synthetic procedures. After said synthetic procedure(s) the amino protecting group as described herein may be selectively removed. Amino protecting groups as known in the art are described generally in T. H. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis, 3rd edition, John Wiley & Sons, New York (1999). Examples of amino protecting groups include, but are not limited to, methoxycarbonyl, t-butoxycarbonyl, 9-fluorenyl-methoxycarbonyl, benzyloxycarbonyl, and the like.

The term “protected amino,” as used herein, refers to an amino group protected with an amino protecting group as defined above.

The term “leaving group” means a functional group or atom which can be displaced by another functional group or atom in a substitution reaction, such as a nucleophilic substitution reaction. By way of example, representative leaving groups include chloro, bromo and iodo groups; sulfonic ester groups, such as mesylate, tosylate, brosylate, nosylate and the like; and acyloxy groups, such as acetoxy, trifluoroacetoxy and the like.

The term “aprotic solvent,” as used herein, refers to a solvent that is relatively inert to proton activity, i.e., not acting as a proton-donor. Examples include, but are not limited to, hydrocarbons, such as hexane and toluene, for example, halogenated hydrocarbons, such as, for example, methylene chloride, ethylene chloride, chloroform, and the like, heterocyclic compounds, such as, for example, tetrahydrofuran and N-methylpyrrolidinone, and ethers such as diethyl ether, bis-methoxymethyl ether. Such compounds are well known to those skilled in the art, and it will be obvious to those skilled in the art that individual solvents or mixtures thereof may be preferred for specific compounds and reaction conditions, depending upon such factors as the solubility of reagents, reactivity of reagents and preferred temperature ranges, for example. Further discussions of aprotic solvents may be found in organic chemistry textbooks or in specialized monographs, for example: Organic Solvents Physical Properties and Methods of Purification, 4th ed., edited by John A. Riddick et al., Vol. II, in the Techniques of Chemistry Series, John Wiley & Sons, N Y, 1986.

The term “protic solvent,” as used herein, refers to a solvent that tends to provide protons, such as an alcohol, for example, methanol, ethanol, propanol, isopropanol, butanol, t-butanol, and the like. Such solvents are well known to those skilled in the art, and it will be obvious to those skilled in the art that individual solvents or mixtures thereof may be preferred for specific compounds and reaction conditions, depending upon such factors as the solubility of reagents, reactivity of reagents and preferred temperature ranges, for example. Further discussions of protogenic solvents may be found in organic chemistry textbooks or in specialized monographs, for example: Organic Solvents Physical Properties and Methods of Purification, 4th ed., edited by John A. Riddick et al., Vol. II, in the Techniques of Chemistry Series, John Wiley & Sons, N Y, 1986.

Combinations of substituents and variables envisioned by this invention are only those that result in the formation of stable compounds. The term “stable,” as used herein, refers to compounds which possess stability sufficient to allow manufacture and which maintains the integrity of the compound for a sufficient period of time to be useful for the purposes detailed herein (e.g., therapeutic or prophylactic administration to a subject).

The synthesized compounds can be separated from a reaction mixture and further purified by a method such as column chromatography, high pressure liquid chromatography, or recrystallization. As can be appreciated by the skilled artisan, further methods of synthesizing the compounds of the Formula herein will be evident to those of ordinary skill in the art. Additionally, the various synthetic steps may be performed in an alternate sequence or order to give the desired compounds. Synthetic chemistry transformations and protecting group methodologies (protection and deprotection) useful in synthesizing the compounds described herein are known in the art and include, for example, those such as described in R. Larock, Comprehensive Organic Transformations, 2^(nd) Ed. Wiley-VCH (1999); T. W. Greene and P. G. M. Wuts, Protective Groups in Organic Synthesis, 3rd Ed., John Wiley and Sons (1999); L. Fieser and M. Fieser, Fieser and Fieser's Reagents for Organic Synthesis, John Wiley and Sons (1994); and L. Paquette, ed., Encyclopedia of Reagents for Organic Synthesis, John Wiley and Sons (1995), and subsequent editions thereof.

The term “subject,” as used herein, refers to an animal. Preferably, the animal is a mammal. More preferably, the mammal is a human. A subject also refers to, for example, dogs, cats, horses, cows, pigs, guinea pigs, fish, birds and the like.

The compounds of this invention may be modified by appending appropriate functionalities to enhance selective biological properties. Such modifications are known in the art and may include those which increase biological penetration into a given biological system (e.g., blood, lymphatic system, central nervous system), increase oral availability, increase solubility to allow administration by injection, alter metabolism and alter rate of excretion.

The compounds described herein contain one or more asymmetric centers and thus give rise to enantiomers, diastereomers, and other stereoisomeric forms that may be defined, in terms of absolute stereochemistry, as (R)- or (S)-, or as (D)- or (L)- for amino acids. The present invention is meant to include all such possible isomers, as well as their racemic and optically pure forms. Optical isomers may be prepared from their respective optically active precursors by the procedures described above, or by resolving the racemic mixtures. The resolution can be carried out in the presence of a resolving agent, by chromatography or by repeated crystallization or by some combination of these techniques which are known to those skilled in the art. Further details regarding resolutions can be found in Jacques, et al., Enantiomers, Racemates, and Resolutions (John Wiley & Sons, 1981). When the compounds described herein contain olefinic double bonds, other unsaturation, or other centers of geometric asymmetry, and unless specified otherwise, it is intended that the compounds include both E and Z geometric isomers or cis- and trans-isomers. Likewise, all tautomeric forms are also intended to be included. Tautomers may be in cyclic or acyclic. The configuration of any carbon-carbon double bond appearing herein is selected for convenience only and is not intended to designate a particular configuration unless the text so states; thus a carbon-carbon double bond or carbon-heteroatom double bond depicted arbitrarily herein as trans may be cis, trans, or a mixture of the two in any proportion.

Certain compounds of the present invention may also exist in different stable conformational forms which may be separable. Torsional asymmetry due to restricted rotation about an asymmetric single bond, for example because of steric hindrance or ring strain, may permit separation of different conformers. The present invention includes each conformational isomer of these compounds and mixtures thereof.

As used herein, the term “pharmaceutically acceptable salt,” refers to those salts which are, within the scope of sound medical judgment, suitable for use in contact with the tissues of humans and lower animals without undue toxicity, irritation, allergic response and the like, and are commensurate with a reasonable benefit/risk ratio. Pharmaceutically acceptable salts are well known in the art. For example, S. M. Berge, et al. describes pharmaceutically acceptable salts in detail in J. Pharmaceutical Sciences, 66: 1-19 (1977). The salts can be prepared in situ during the final isolation and purification of the compounds of the invention, or separately by reacting the free base function with a suitable organic acid. Examples of pharmaceutically acceptable salts include, but are not limited to, nontoxic acid addition salts are salts of an amino group formed with inorganic acids such as hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid and perchloric acid or with organic acids such as acetic acid, maleic acid, tartaric acid, citric acid, succinic acid or malonic acid or by using other methods used in the art such as ion exchange. Other pharmaceutically acceptable salts include, but are not limited to, adipate, alginate, ascorbate, aspartate, benzenesulfonate, benzoate, bisulfate, borate, butyrate, camphorate, camphorsulfonate, citrate, cyclopentane-propionate, digluconate, dodecylsulfate, ethanesulfonate, formate, fumarate, glucoheptonate, glycerophosphate, gluconate, hemisulfate, heptanoate, hexanoate, hydroiodide, 2-hydroxy-ethanesulfonate, lactobionate, lactate, laurate, lauryl sulfate, malate, maleate, malonate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, nitrate, oleate, oxalate, palmitate, pamoate, pectinate, persulfate, 3-phenylpropionate, phosphate, picrate, pivalate, propionate, stearate, succinate, sulfate, tartrate, thiocyanate, p-toluenesulfonate, undecanoate, valerate salts, and the like. Representative alkali or alkaline earth metal salts include sodium, lithium, potassium, calcium, magnesium, and the like. Further pharmaceutically acceptable salts include, when appropriate, nontoxic ammonium, quaternary ammonium, and amine cations formed using counterions such as halide, hydroxide, carboxylate, sulfate, phosphate, nitrate, alkyl having from 1 to 6 carbon atoms, sulfonate and aryl sulfonate.

As used herein, the term “pharmaceutically acceptable ester” refers to esters which hydrolyze in vivo and include those that break down readily in the human body to leave the parent compound or a salt thereof. Suitable ester groups include, for example, those derived from pharmaceutically acceptable aliphatic carboxylic acids, particularly alkanoic, alkenoic, cycloalkanoic and alkanedioic acids, in which each alkyl or alkenyl moiety advantageously has not more than 6 carbon atoms. Examples of particular esters include, but are not limited to, formates, acetates, propionates, butyrates, acrylates and ethylsuccinates.

Pharmaceutical Compositions

The pharmaceutical compositions of the present invention comprise a therapeutically effective amount of a compound of the present invention formulated together with one or more pharmaceutically acceptable carriers or excipients.

As used herein, the term “pharmaceutically acceptable carrier or excipient” means a non-toxic, inert solid, semi-solid or liquid filler, diluent, encapsulating material or formulation auxiliary of any type. Some examples of materials which can serve as pharmaceutically acceptable carriers are sugars such as lactose, glucose and sucrose; starches such as corn starch and potato starch; cellulose and its derivatives such as sodium carboxymethyl cellulose, ethyl cellulose and cellulose acetate; powdered tragacanth; malt; gelatin; talc; excipients such as cocoa butter and suppository waxes; oils such as peanut oil, cottonseed oil, safflower oil, sesame oil, olive oil, corn oil and soybean oil; glycols such as propylene glycol; esters such as ethyl oleate and ethyl laurate; agar; buffering agents such as magnesium hydroxide and aluminum hydroxide; alginic acid; pyrogen-free water; isotonic saline; Ringer's solution; ethyl alcohol, and phosphate buffer solutions, as well as other non-toxic compatible lubricants such as sodium lauryl sulfate and magnesium stearate, as well as coloring agents, releasing agents, coating agents, sweetening, flavoring and perfuming agents, preservatives and antioxidants can also be present in the composition, according to the judgment of the formulator.

The pharmaceutical compositions of this invention may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir, preferably by oral administration or administration by injection. The pharmaceutical compositions of this invention may contain any conventional non-toxic pharmaceutically-acceptable carriers, adjuvants or vehicles. In some cases, the pH of the formulation may be adjusted with pharmaceutically acceptable acids, bases or buffers to enhance the stability of the formulated compound or its delivery form. The term parenteral as used herein includes subcutaneous, intracutaneous, intravenous, intramuscular, intraarticular, intra-arterial, intrasynovial, intrasternal, intrathecal, intralesional and intracranial injection or infusion techniques.

Liquid dosage forms for oral administration include pharmaceutically acceptable emulsions, microemulsions, solutions, suspensions, syrups and elixirs. In addition to the active compounds, the liquid dosage forms may contain inert diluents commonly used in the art such as, for example, water or other solvents, solubilizing agents and emulsifiers such as ethyl alcohol, isopropyl alcohol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1,3-butylene glycol, dimethylformamide, oils (in particular, cottonseed, groundnut, corn, germ, olive, castor, and sesame oils), glycerol, tetrahydrofurfuryl alcohol, polyethylene glycols and fatty acid esters of sorbitan, and mixtures thereof. Besides inert diluents, the oral compositions can also include adjuvants such as wetting agents, emulsifying and suspending agents, sweetening, flavoring, and perfuming agents.

Injectable preparations, for example, sterile injectable aqueous or oleaginous suspensions, may be formulated according to the known art using suitable dispersing or wetting agents and suspending agents. The sterile injectable preparation may also be a sterile injectable solution, suspension or emulsion in a nontoxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol. Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution, U.S.P. and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose, any bland fixed oil can be employed including synthetic mono- or diglycerides. In addition, fatty acids such as oleic acid are used in the preparation of injectable.

The injectable formulations can be sterilized, for example, by filtration through a bacterial-retaining filter, or by incorporating sterilizing agents in the form of sterile solid compositions which can be dissolved or dispersed in sterile water or other sterile injectable medium prior to use.

In order to prolong the effect of a drug, it is often desirable to slow the absorption of the drug from subcutaneous or intramuscular injection. This may be accomplished by the use of a liquid suspension of crystalline or amorphous material with poor water solubility. The rate of absorption of the drug then depends upon its rate of dissolution, which, in turn, may depend upon crystal size and crystalline form. Alternatively, delayed absorption of a parenterally administered drug form is accomplished by dissolving or suspending the drug in an oil vehicle. Injectable depot forms are made by forming microencapsule matrices of the drug in biodegradable polymers such as polylactide-polyglycolide. Depending upon the ratio of drug to polymer and the nature of the particular polymer employed, the rate of drug release can be controlled. Examples of other biodegradable polymers include poly(orthoesters) and poly(anhydrides). Depot injectable formulations are also prepared by entrapping the drug in liposomes or microemulsions that are compatible with body tissues.

Compositions for rectal or vaginal administration are preferably suppositories which can be prepared by mixing the compounds of this invention with suitable non-irritating excipients or carriers such as cocoa butter, polyethylene glycol or a suppository wax which are solid at ambient temperature but liquid at body temperature and therefore melt in the rectum or vaginal cavity and release the active compound.

Solid dosage forms for oral administration include capsules, tablets, pills, powders, and granules. In such solid dosage forms, the active compound is mixed with at least one inert, pharmaceutically acceptable excipient or carrier such as sodium citrate or dicalcium phosphate and/or: a) fillers or extenders such as starches, lactose, sucrose, glucose, mannitol, and silicic acid, b) binders such as, for example, carboxymethylcellulose, alginates, gelatin, polyvinylpyrrolidinone, sucrose, and acacia, c) humectants such as glycerol, d) disintegrating agents such as agar-agar, calcium carbonate, potato or tapioca starch, alginic acid, certain silicates, and sodium carbonate, e) solution retarding agents such as paraffin, f) absorption accelerators such as quaternary ammonium compounds, g) wetting agents such as, for example, cetyl alcohol and glycerol monostearate, h) absorbents such as kaolin and bentonite clay, and i) lubricants such as talc, calcium stearate, magnesium stearate, solid polyethylene glycols, sodium lauryl sulfate, and mixtures thereof. In the case of capsules, tablets and pills, the dosage form may also comprise buffering agents.

Solid compositions of a similar type may also be employed as fillers in soft and hard-filled gelatin capsules using such excipients as lactose or milk sugar as well as high molecular weight polyethylene glycols and the like.

The solid dosage forms of tablets, dragees, capsules, pills, and granules can be prepared with coatings and shells such as enteric coatings and other coatings well known in the pharmaceutical formulating art. They may optionally contain opacifying agents and can also be of a composition that they release the active ingredient(s) only, or preferentially, in a certain part of the intestinal tract, optionally, in a delayed manner. Examples of embedding compositions that can be used include polymeric substances and waxes.

Dosage forms for topical or transdermal administration of a compound of this invention include ointments, pastes, creams, lotions, gels, powders, solutions, sprays, inhalants or patches. The active component is admixed under sterile conditions with a pharmaceutically acceptable carrier and any needed preservatives or buffers as may be required. Ophthalmic formulation, ear drops, eye ointments, powders and solutions are also contemplated as being within the scope of this invention.

The ointments, pastes, creams and gels may contain, in addition to an active compound of this invention, excipients such as animal and vegetable fats, oils, waxes, paraffins, starch, tragacanth, cellulose derivatives, polyethylene glycols, silicones, bentonites, silicic acid, talc and zinc oxide, or mixtures thereof.

Powders and sprays can contain, in addition to the compounds of this invention, excipients such as lactose, talc, silicic acid, aluminum hydroxide, calcium silicates and polyamide powder, or mixtures of these substances. Sprays can additionally contain customary propellants such as chlorofluorohydrocarbons.

Transdermal patches have the added advantage of providing controlled delivery of a compound to the body. Such dosage forms can be made by dissolving or dispensing the compound in the proper medium. Absorption enhancers can also be used to increase the flux of the compound across the skin. The rate can be controlled by either providing a rate controlling membrane or by dispersing the compound in a polymer matrix or gel.

For pulmonary delivery, a therapeutic composition of the invention is formulated and administered to the patient in solid or liquid particulate form by direct administration e.g., inhalation into the respiratory system. Solid or liquid particulate forms of the active compound prepared for practicing the present invention include particles of respirable size: that is, particles of a size sufficiently small to pass through the mouth and larynx upon inhalation and into the bronchi and alveoli of the lungs. Delivery of aerosolized therapeutics, particularly aerosolized antibiotics, is known in the art (see, for example U.S. Pat. No. 5,767,068 to Van Devanter et al., U.S. Pat. No. 5,508,269 to Smith et al., and WO 98/43650 by Montgomery, all of which are incorporated herein by reference).

Combination and Alternation Therapy

It has been recognized that drug-resistant variants of HIV, HBV and HCV can emerge after prolonged treatment with an antiviral agent. Drug resistance most typically occurs by mutation of a gene that encodes for a protein such as an enzyme used in viral replication, and most typically in the case of HIV, reverse transcriptase, protease, or DNA polymerase, and in the case of HBV, DNA polymerase, or in the case of HCV, RNA polymerase, protease, or helicase. Recently, it has been demonstrated that the efficacy of a drug against HIV infection can be prolonged, augmented, or restored by administering the compound in combination or alternation with a second, and perhaps third, antiviral compound that induces a different mutation from that caused by the principle drug. The compounds can be used for combination are selected from the group consisting of a HBV polymerase inhibitor, interferon, TLR modulators such as TLR-7 agonists or TLR-9 agonists, therapeutic vaccines, immune activator of certain cellular viral RNA sensors, viral entry inhibitor, viral maturation inhibitor, distinct capsid assembly modulator, antiviral compounds of distinct or unknown mechanism, and combination thereof. Alternatively, the pharmacokinetics, biodistribution, or other parameter of the drug can be altered by such combination or alternation therapy. In general, combination therapy is typically preferred over alternation therapy because it induces multiple simultaneous stresses on the virus.

Preferred compounds for combination or alternation therapy for the treatment of HBV include 3TC, FTC, L-FMAU, interferon, adefovir dipivoxil, entecavir, telbivudine (L-dT), valtorcitabine (3′-valinyl L-dC), β-D-dioxolanyl-guanine (DXG), β-D-dioxolanyl-2,6-diaminopurine (DAPD), and β-D-dioxolanyl-6-chloropurine (ACP), famciclovir, penciclovir, lobucavir, ganciclovir, and ribavirin.

Although the invention has been described with respect to various preferred embodiments, it is not intended to be limited thereto, but rather those skilled in the art will recognize that variations and modifications may be made therein which are within the spirit of the invention and the scope of the appended claims.

Antiviral Activity

An inhibitory amount or dose of the compounds of the present invention may range from about 0.01 mg/Kg to about 500 mg/Kg, alternatively from about 1 to about 50 mg/Kg. Inhibitory amounts or doses will also vary depending on route of administration, as well as the possibility of co-usage with other agents.

According to the methods of treatment of the present invention, viral infections, conditions are treated or prevented in a patient such as a human or another animal by administering to the patient a therapeutically effective amount of a compound of the invention, in such amounts and for such time as is necessary to achieve the desired result.

By a “therapeutically effective amount” of a compound of the invention is meant an amount of the compound which confers a therapeutic effect on the treated subject, at a reasonable benefit/risk ratio applicable to any medical treatment. The therapeutic effect may be objective (i.e., measurable by some test or marker) or subjective (i.e., subject gives an indication of or feels an effect). An effective amount of the compound described above may range from about 0.1 mg/Kg to about 500 mg/Kg, preferably from about 1 to about 50 mg/Kg. Effective doses will also vary depending on route of administration, as well as the possibility of co-usage with other agents. It will be understood, however, that the total daily usage of the compounds and compositions of the present invention will be decided by the attending physician within the scope of sound medical judgment. The specific therapeutically effective dose level for any particular patient will depend upon a variety of factors including the disorder being treated and the severity of the disorder; the activity of the specific compound employed; the specific composition employed; the age, body weight, general health, sex and diet of the patient; the time of administration, route of administration, and rate of excretion of the specific compound employed; the duration of the treatment; drugs used in combination or contemporaneously with the specific compound employed; and like factors well known in the medical arts.

The total daily dose of the compounds of this invention administered to a human or other animal in single or in divided doses can be in amounts, for example, from 0.01 to 50 mg/kg body weight or more usually from 0.1 to 25 mg/kg body weight. Single dose compositions may contain such amounts or submultiples thereof to make up the daily dose. In general, treatment regimens according to the present invention comprise administration to a patient in need of such treatment from about 10 mg to about 1000 mg of the compound(s) of this invention per day in single or multiple doses.

The compounds of the present invention described herein can, for example, be administered by injection, intravenously, intra-arterial, subdermally, intraperitoneally, intramuscularly, or subcutaneously; or orally, buccally, nasally, transmucosally, topically, in an ophthalmic preparation, or by inhalation, with a dosage ranging from about 0.1 to about 500 mg/kg of body weight, alternatively dosages between 1 mg and 1000 mg/dose, every 4 to 120 hours, or according to the requirements of the particular drug. The methods herein contemplate administration of an effective amount of compound or compound composition to achieve the desired or stated effect. Typically, the pharmaceutical compositions of this invention will be administered from about 1 to about 6 times per day or alternatively, as a continuous infusion. Such administration can be used as a chronic or acute therapy. The amount of active ingredient that may be combined with pharmaceutically excipients or carriers to produce a single dosage form will vary depending upon the host treated and the particular mode of administration. A typical preparation will contain from about 5% to about 95% active compound (w/w). Alternatively, such preparations may contain from about 20% to about 80% active compound.

Lower or higher doses than those recited above may be required. Specific dosage and treatment regimens for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health status, sex, diet, time of administration, rate of excretion, drug combination, the severity and course of the disease, condition or symptoms, the patient's disposition to the disease, condition or symptoms, and the judgment of the treating physician.

Upon improvement of a patient's condition, a maintenance dose of a compound, composition or combination of this invention may be administered, if necessary. Subsequently, the dosage or frequency of administration, or both, may be reduced, as a function of the symptoms, to a level at which the improved condition is retained when the symptoms have been alleviated to the desired level. Patients may, however, require intermittent treatment on a long-term basis upon any recurrence of disease symptoms.

When the compositions of this invention comprise a combination of a compound of the Formula described herein and one or more additional therapeutic or prophylactic agents, both the compound and the additional agent should be present at dosage levels of between about 1 to 100%, and more preferably between about 5 to 95% of the dosage normally administered in a monotherapy regimen. The additional agents may be administered separately, as part of a multiple dose regimen, from the compounds of this invention. Alternatively, those agents may be part of a single dosage form, mixed together with the compounds of this invention in a single composition.

The said “additional therapeutic or prophylactic agents” includes but not limited to, immune therapies (e.g. interferon), therapeutic vaccines, antifibrotic agents, anti-inflammatory agents such as corticosteroids or NSAIDs, bronchodilators such as beta-2 adrenergic agonists and xanthines (e.g. theophylline), mucolytic agents, anti-muscarinics, anti-leukotrienes, inhibitors of cell adhesion (e.g. ICAM antagonists), anti-oxidants (e.g. N-acetylcysteine), cytokine agonists, cytokine antagonists, lung surfactants and/or antimicrobial and anti-viral agents (e.g. ribavirin and amantidine). The compositions according to the invention may also be used in combination with gene replacement therapy.

Abbreviations

Abbreviations which may be used in the descriptions of the scheme and the examples that follow are: Ac for acetyl; AcOH for acetic acid; AIBN for azobisisobutyronitrile; BINAP for 2,2′-bis(diphenylphosphino)-1,1′-binaphthyl; Boc₂O for di-tert-butyl-dicarbonate; Boc for t-butoxycarbonyl; Bpoc for 1-methyl-1-(4-biphenylyl)ethyl carbonyl; Bz for benzoyl; Bn for benzyl; BocNHOH for tert-butyl N-hydroxycarbamate; t-BuOK for potassium tert-butoxide; Bu₃SnH for tributyltin hydride; BOP for (benzotriazol-1-yloxy)tris(dimethylamino)phospho-nium Hexafluorophosphate; Brine for sodium chloride solution in water; BSA for N,O-bis(trimethylsilyl)acetamide; CDI for carbonyldiimidazole; DCM or CH₂C₂ for dichloro-methane; CH₃ for methyl; CH₃CN or ACN for acetonitrile; Cs₂CO₃ for cesium carbonate; CuCl for copper (I) chloride; CuI for copper (I) iodide; dba for dibenzylidene acetone; dppb for diphenylphos-phinobutane; DBU for 1,8-diazabicyclo[5.4.0]-undec-7-ene; DCC for N,N′-dicyclohexyl-carbodiimide; DEAD for diethylazodicarboxylate; DIAD for diisopropyl azodicarboxylate; DIPEA or (i-Pr)₂EtN for N,N,-diisopropylethyl amine; Dess-Martin periodinane for 1,1,1-tris(acetyloxy)-1,1-dihydro-1,2-benziodoxol-3-(1H)-one; DMAP for 4-dimethylamino-pyridine; DCE for 1,2-dichloroethane; DMA for N,N-dimethylacetamide; DME for 1,2-dimethoxyethane; DMF for N,N-dimethylformamide; DMSO for dimethyl sulfoxide; DMT for di(p-methoxyphenyl)-phenylmethyl or dimethoxy-trityl; DPPA for diphenylphosphoryl azide; EDC for N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide; EDC HCl for N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride; EtOAc or EA for ethyl acetate; EtOH for ethanol; Et₂O for diethyl ether; HATU for O-(7-azabenzotriazol-1-yl)-N,N,N′,N′,-tetramethyluronium Hexafluoro-phosphate; HCl for hydrogen chloride; HOBT for 1-hydroxybenzotriazole; K₂CO₃ for potassium carbonate; n-BuLi for n-butyl lithium; i-BuLi for i-butyl lithium; t-BuLi for t-butyl lithium; DDQ for 2,3-dichloro-5,6-dicyano-1,4-benzoquinone; PhLi for phenyl lithium; LDA for lithium diisopropylamide; LiTMP for lithium 2,2,6,6-tetramethyl-piperidinate; mCPBA or m-CPBA for meta-chloroperbenzoic acid; MeOH for methanol; Mg for magnesium; MOM for methoxymethyl; Ms for mesyl or —SO₂—CH₃; Ms₂O for methanesulfonic anhydride or mesyl-anhydride; MTBE for t-butyl methyl ether; NaN(TMS)₂ or NaHMDS for sodium bis(trimethylsilyl)amide; NaCl for sodium chloride; NaH for sodium hydride; NaHCO₃ for sodium bicarbonate or sodium hydrogen carbonate; Na₂CO₃ sodium carbonate; NaOH for sodium hydroxide; Na₂SO₄ for sodium sulfate; NaHSO₃ for sodium bisulfite or sodium hydrogen sulfite; Na₂S₂O₃ for sodium thiosulfate; NH₂NH₂ for hydrazine; NH₄HCO₃ for ammonium bicarbonate; NH₄Cl for ammonium chloride; NMO for N-methylmorpholine N-oxide; NaIO₄ for sodium periodate; Ni for nickel; OH for hydroxyl; OsO₄ for osmium tetroxide; PPA for polyphophoric acid; p-TSA or PTSA forp-toluenesulfonic acid; PPTS for pyridinium p-toluenesulfonate; PhI(OPiv)₂ for Bis(tert-butylcarbonyloxy)iodobenzene; Rh₂(Esp)₂ for Bis[rhodium(α,α,α′,α′-tetramethyl-1,3-benzenedipropionic acid)]; TBAF for tetrabutylammonium fluoride; TEA or Et₃N for triethylamine; TES for triethylsilyl; TESCI for triethylsilyl chloride; TESOTf for triethylsilyl trifluoromethanesulfonate; TFA for trifluoroacetic acid; THE for tetrahydrofuran; TMEDA for N,N,N′,N′-tetramethylethylene-diamine; TPP or PPh₃ for triphenyl-phosphine; Troc for 2,2,2-trichloroethyl carbonyl; Ts for tosyl or —SO₂—C₆H₄CH₃; Ts₂O for tolylsulfonic anhydride or tosyl-anhydride; TsOH for p-tolylsulfonic acid; Tf for triflate; LCMS for liquid chromatography mass spectrometry; TLC for thin layer chromatography; PE for petroleum ether; TFAA for trifluoroacetic anhydride; THP for tetrahydropyranyl; MS for mass spectrometry; ESI for electrospray ionization; RPHPLC for reverse phase high performance liquid chromatography; Pd for palladium; Ph for phenyl; POPd for dihydrogen dichlorobis(di-tert-butylphosphinito-xP)palladate(II); Pd₂(dba)₃ for tris(diben-zylideneacetone) dipalladium (0); Pd(PPh₃)₄ for tetrakis(triphenylphosphine)-palladium (0); PdCl₂(PPh₃)₂ for trans-dichlorobis-(triphenylphosphine)palladium (II); Pt for platinum; Rh for rhodium; rt for room temperature; Ru for ruthenium; TBS for tert-butyl dimethylsilyl; TMS for trimethylsilyl; or TMSCI for trimethylsilyl chloride.

Synthetic Methods

The compounds and processes of the present invention will be better understood in connection with the following synthetic schemes that illustrate the methods by which the compounds of the invention may be prepared. These schemes are of illustrative purpose, and are not meant to limit the scope of the invention. Equivalent, similar, or suitable solvents, reagents or reaction conditions may be substituted for those particular solvents, reagents, or reaction conditions described herein without departing from the general scope of the method of synthesis.

Illustrated in Scheme 1, intermediate compounds such as 1 can be prepared according to the synthetic methods herein, or by similar methods known to those skilled in the art. Compounds such as intermediate 1 (R₁, R₂, R₄, R₅, R₆, R₇, R₈, A, and W as defined previously; X defined as —Cl, —Br, —I, or —OTf) can be reacted directly in a cross-coupling reaction with compounds such as intermediate 2 (R₃ as defined previously; M defined as a boron, tin, or hydrogen atom; Q² defined as —OH, —OR, —R, or halogen; n equal to 0, 1, 2, or 3) whereby intermediate 2 is commercially available or can be prepared by those familiar with the skill of the arts. The stated cross-coupling reaction can be mediated by a metal-based reagent (denoted as [Metal] in Scheme 1) including, but not limited to: Pd(OAc)₂, PdCl₂(dppf), Pd(Ph₃P)₄, Pd₂(dba)₃, PdRuPhos G2, or Pd^(t)BuXPhos G3. The stated cross-coupling reaction can also be mediated by a base (denoted as [Base] in Scheme 1) including, but not limited to: Cs₂CO₃, K₂CO₃, Na₂CO₃, CsF, KOAc, K₃PO₄, Et₃N, or DBU. The stated cross-coupling reaction can be performed in a solvent or a mixture of solvents (denoted as [Solvent] in Scheme 1) including, but not limited to: THF, toluene, benzene, DMF, DMA, 1,4-dioxane, or water. The stated cross-coupling reaction can be performed at a temperature range between 0° C. and 180° C. where appropriate.

Illustrated in Scheme 2, intermediate compounds such as 7(R₂, R₃, and A as defined previously; X defined as —Cl, —Br, —I, or —OTf) can be produced in a two-step sequence outlined herein, or by other methods known to those skilled in the art. More specifically, compounds such as intermediate 4 (L defined as —OH, —NH₂, or —SH) can be reacted with acetal 5 (R as defined previously), a base (denoted as [Base] in Scheme 2) including, but not limited to: Cs₂CO₃, NaH, or Et₃N, in a solvent (denoted as [Solvent] in Scheme 2) including, but not limited to: toluene, DMF, or 1,4-dioxane to form intermediate 6. This can be reacted with an acidic reagent (denoted as [Acid] in Scheme 2) including, but not limited to: polyphosphoric acid, sulfuric acid, or p-TsOH, in a solvent (denoted as [Solvent] in Scheme 2) including, but not limited to: chlorobenzene, xylene, or toluene to produce 7.

Illustrated in Scheme 3, intermediate compounds such as 7 (R₂, R₃, and A as defined previously; X defined as —Cl, —Br, —I, or —OTf) can be produced in a two-step sequence outlined herein, or by other methods known to those skilled in the art. More specifically, compounds such as intermediate 8 (L defined as —OH, —NH₂, or —SH) can be reacted with acetal 9 (R as defined previously), a base (denoted as [Base] in Scheme 3) including, but not limited to: Cs₂CO₃, NaH, or Et₃N, in a solvent (denoted as [Solvent] in Scheme 3) including, but not limited to: toluene, DMF, or 1,4-dioxane to form intermediate 10. This can be reacted with an acidic reagent (denoted as [Acid] in Scheme 3) including, but not limited to: polyphosphoric acid, sulfuric acid, or p-TsOH, in a solvent (denoted as [Solvent] in Scheme 3) including, but not limited to: chlorobenzene, xylene, or toluene to produce 7.

Illustrated in Scheme 4, intermediate compounds such as 18 (R₁, R₂, R₃, R₄, R₅, R₆, R₇, R₈, and W as defined previously) can be produced in the sequence outlined herein, or by other methods known to those skilled in the art. More specifically, compounds such as intermediate intermediate 7 can be reacted in an enolate coupling reaction with intermediate 11, whereby intermediate 11 is commercially available or can be prepared by those familiar with the skill of the arts, to form intermediate 12. This can be reacted in a reductive amination reaction to form amine 13. This can be reacted in a formylation reaction to provide intermediate 14. This can undergo an intramolecular cyclization reaction (denoted as [Cyclization] in Scheme 4) mediated by an electrophilic reagent, or mixture of reagents including, but not limited to: POCl₃, FeCl₃, MsOH, (COCl)₂, or SOCl₂ to produce imine 15. This can be reacted in a cyclocondensation reaction with ketone to provide intermediate 17. This can undergo an oxidation reaction (denoted as [Oxidation] in Scheme 4) mediated by an oxidant including, but not limited to: 12, DDQ, or p-chloranil followed by hydrolysis and chiral purification via SFC to produce pyridone 18.

All references cited herein, whether in print, electronic, computer readable storage media or other form, are expressly incorporated by reference in their entirety, including but not limited to, abstracts, articles, journals, publications, texts, treatises, internet web sites, databases, patents, and patent publications.

Various changes and modifications to the disclosed embodiments will be apparent to those skilled in the art and such changes and modifications including, without limitation, those relating to the chemical structures, substituents, derivatives, formulations and/or methods of the invention may be made without departing from the spirit of the invention and the scope of the appended claims.

Although the invention has been described with respect to various preferred embodiments, it is not intended to be limited thereto, but rather those skilled in the art will recognize that variations and modifications may be made therein which are within the spirit of the invention and the scope of the appended claims.

EXAMPLES

The compounds and processes of the present invention will be better understood in connection with the following examples, which are intended as an illustration only and not limiting of the scope of the invention.

General Conditions:

Mass spectra were run on LC-MS systems using electrospray ionization. These were Agilent 1290 Infinity II systems with an Agilent 6120 Quadrupole detector. Spectra were obtained using a ZORBAX Eclipse XDB-C18 column (4.6×30 mm, 1.8 micron). Spectra were obtained at 298K using a mobile phase of 0.1% formic acid in water (A) and 0.1% formic acid in acetonitrile (B). Spectra were obtained with the following solvent gradient: 5% (B) from 0-1.5 min, 5-95% (B) from 1.5-4.5 min, and 95% (B) from 4.5-6 min. The solvent flowrate was 1.2 mL/min. Compounds were detected at 210 nm and 254 nm wavelengths. [M+H]⁺ refers to mono-isotopic molecular weights.

NMR spectra were run on a Bruker 400 MHz spectrometer. Spectra were measured at 298K and referenced using the solvent peak. Chemical shifts for ¹H NMR are reported in parts per million (ppm).

Compounds were purified via reverse-phase high-performance liquid chromatography (RPHPLC) using a Gilson GX-281 automated liquid handling system. Compounds were purified on a Phenomenex Kinetex EVO C18 column (250×21.2 mm, 5 micron), unless otherwise specified. Compounds were purified at 298K using a mobile phase of water (A) and acetonitrile (B) using gradient elution between 0% and 100% (B), unless otherwise specified. The solvent flowrate was 20 mL/min and compounds were detected at 254 nm wavelength.

Alternatively, compounds were purified via normal-phase liquid chromatography (NPLC) using a Teledyne ISCO Combiflash purification system. Compounds were purified on a REDISEP silica gel cartridge. Compounds were purified at 298K and detected at 254 nm wavelength.

Example 1: Synthesis of (S)-5-(tert-butyl)-12-chloro-9-oxo-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

Step 1: Into a 1 L round bottom flask was placed 5-bromo-2-chlorophenol (50 g), 2-bromo-1,1-diethoxyethane (55 g), K₂CO₃ (50 g) and DMF (500 mL). The mixture was warmed to 80° C. and stirred for 18 h. The mixture was diluted with water and extracted with EA. The combined organic layers were dried, filtered and concentrated. The residue was purified by silica gel chromatography to afford 4-bromo-1-chloro-2-(2,2-diethoxyethoxy)benzene (50 g, 64% yield).

Step 2: Into a 1 L round bottom flask was added 4-bromo-1-chloro-2-(2,2-diethoxyethoxy)benzene (50 g) and 500 mL of chlorobenzene. Polyphosphoric acid (50 g) was added portionwise. The mixture was heated to 130° C. and stirred for 3 hours. The mixture was quenched with aq. NaHCO₃ and concentrated. The residue was extracted with EA and the combined organic layers were dried, filtered and concentrated. The crude material was purified by silica gel chromatography to afford 4-bromo-7-chlorobenzofuran (28 g, 78% yield).

Step 3: Into a 1 L three-necked round bottom flask was added 4-bromo-7-chlorobenzofuran (28 g), t-butyl methyl ketone (36 g), Pd₂(dba)₃.CHCl₃ (6.2 g), Xantphos (6.9 g), t-BuONa (34.5 g) and THE (300 mL) under a nitrogen atmosphere. The mixture was heated to 85° C. and stirred for 5 hours. The mixture was quenched with aq. NH₄Cl and extracted with EA. The combined organic layers were dried, filtered and concentrated. The residue was purified by silica gel chromatography to afford 1-(7-chlorobenzofuran-4-yl)-3,3-dimethylbutan-2-one (28 g, 93% yield).

Step 4: Into a 1 L round bottom flask was added 1-(7-chlorobenzofuran-4-yl)-3,3-dimethylbutan-2-one (28 g), NH₄OAc (84 g) and MeOH (500 mL). The mixture was stirred for 30 min at ambient temperature. Then NaBH₃CN (27 g) was added portionwise. The resulting mixture was refluxed for 18 h, then the solvent was evaporated. The residue was washed with 3N NaOH and extracted with EA. The combined organic layers were dried, filtered and concentrated. The crude 1-(7-chlorobenzofuran-4-yl)-3,3-dimethylbutan-2-amine (20 g) was directly used in next step without purification.

Step 5: Into a 500 mL round bottom flask was added crude 1-(7-chlorobenzofuran-4-yl)-3,3-dimethylbutan-2-amine (20 g), and 200 mL of 1,4-dioxane. Then, formic acid (14.7 g) was added. The new mixture was stirred at 100° C. for 5 hours. The mixture was concentrated and the resulting crude N-(1-(7-chlorobenzofuran-4-yl)-3,3-dimethylbutan-2-yl)formamide (15 g) was directly used in next step without purification.

Step 6: Into a 500 mL round bottom flask was placed crude N-(1-(7-chlorobenzofuran-4-yl)-3,3-dimethylbutan-2-yl)formamide (15 g) and 300 mL of anhydrous DCM. Oxalyl chloride (7.5 g) was added dropwise at room temperature under a nitrogen atmosphere. Then the mixture was cooled to −10° C. Anhydrous FeCl₃ (10.4 g) was added portionwise. The reaction was slowly warmed to ambient temperature and stirred for 18 h. The reaction was quenched with 2N HCl. The organic layers was washed with brine solution, dried, filtered and concentrated. The residue was dissolved in concentrated H₂SO₄/MeOH (1/19, 150 mL) and stirred at 70° C. for 2 hours. The mixture was cooled and concentrated. The residue was basified by aq. NaHCO₃ and extracted with DCM. The combined organic layers were dried, filtered and concentrated to afford crude 8-(tert-butyl)-4-chloro-8,9-dihydrofuro[3,2-f]isoquinoline (10 g), which was used in next step without further purification.

Step 7: Into a 500 mL round bottom flask was added crude 8-(tert-butyl)-4-chloro-8,9-dihydrofuro[3,2-f]isoquinoline (10 g) and EtOH/H₂O (3/1, 150 mL). Then, ethyl 2-(ethoxymethylene)-3-oxobutanoate (21.4 g) was added. The mixture was stirred at 100° C. for 19 h. The mixture was concentrated and purified by silica gel chromatography to afford ethyl 5-(tert-butyl)-12-chloro-9-oxo-4,9,10,10a-tetrahydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylate (5 g, 11% yield for 4 steps).

Step 8: Into a 250 mL round bottom flask was added ethyl 5-(tert-butyl)-12-chloro-9-oxo-4,9,10,10a-tetrahydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylate (5 g) and 50 mL of DME. Then, p-chloranil (6.1 g) was added. The mixture was heated to 70° C. and stirred for 1 hour. The mixture was concentrated and purified by silica gel chromatography to furnish ethyl 5-(tert-butyl)-12-chloro-9-oxo-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylate (3.2 g, 60% yield).

Step 9: Ethyl 5-(tert-butyl)-12-chloro-9-oxo-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylate (120 mg) was dissolved in H₂O/THF/MeOH (4/1/3, 10 mL), then NaOH (20 mg) was added. After stirring at room temperature for 2 h, the volatiles were removed. The aqueous layer was brought to pH=3 by addition of 5N HCl. The product was extracted with DCM, and concentrated. The residue was purified by SFC (Column: CHIRAL ART Cellulose-SB, mobile phase: 20% CO₂/MeOH to provide the product, (S)-5-(tert-butyl)-12-chloro-9-oxo-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid as a white solid (55 mg, 46% yield). ESI MS m/z=372.1 [M+H]*.

Example 2: Synthesis of (S)-5-(tert-butyl)-12-chloro-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

Step 1: Into a 1 L round bottom flask was placed 2-bromo-5-chlorophenol (50 g), 2-bromo-1,1-diethoxyethane (55 g), K₂CO₃ (50 g) and DMF (500 mL). The mixture was warmed to 80° C. and stirred for 14 h. The mixture was diluted with water and extracted with EA. The combined organic layers were dried, filtered and concentrated. The residue was purified by silica gel chromatography to afford 1-bromo-4-chloro-2-(2,2-diethoxyethoxy)benzene (50 g, 64% yield).

Step 2: Into a 1 L round bottom flask was added 1-bromo-4-chloro-2-(2,2-diethoxyethoxy)benzene (70 g) and 500 mL of chlorobenzene. PPA (70 g) was added portionwise. The mixture was heated to 130° C. and stirred for 3 hours. The mixture was quenched with aq. NaHCO₃and concentrated. The residue was extracted with EA and the combined organic layers were dried, filtered and concentrated. The crude material was purified by silica gel chromatography to afford 7-bromo-4-chlorobenzofuran (36 g, 72% yield).

Step 3: Into a 1 L three-necked round bottom flask was added 7-bromo-4-chlorobenzofuran (36 g), t-butyl methyl ketone (48 g), Pd₂(dba)₃-CHCl₃ (8.2 g), Xantphos (9.2 g), t-BuONa (46 g) and THE (500 mL) under nitrogen atmosphere. The mixture was heated to 85° C. and stirred for 5 hours. The mixture was quenched with aq. NH₄Cl and extracted with EA. The combined organic layers were dried, filtered and concentrated. The residue was purified by silica gel chromatography to afford 1-(4-chlorobenzofuran-7-yl)-3,3-dimethylbutan-2-one (38 g, 93% yield).

Step 4: Into a 1 L round bottom flask was added 1-(4-chlorobenzofuran-7-yl)-3,3-dimethylbutan-2-one (38 g), NH₄OAc (114 g) and MeOH (500 mL). The mixture was stirred for 30 min at ambient temperature. Then NaBH₃CN (36.8 g) was added portionwise. The resulting mixture was refluxed for 20 h. The solvent was evaporated. The residue was washed with 3N NaOH and extracted with EA. The combined organic layers were dried, filtered and concentrated. The crude 1-(4-chlorobenzofuran-7-yl)-3,3-dimethylbutan-2-amine (38 g) was directly used in next step without purification.

Step 5: Into a 1 L round bottom flask was added crude 1-(4-chlorobenzofuran-7-yl)-3,3-dimethylbutan-2-amine (38 g) and 400 mL of 1,4-dioxane, then formic acid (28 g) was added. The new mixture was stirred at 100° C. for 5 hours. The mixture was concentrated and the resulting crude N-(1-(4-chlorobenzofuran-7-yl)-3,3-dimethylbutan-2-yl)formamide (35 g) was directly used in next step without purification.

Step 6: Into a 500 mL round bottom flask was placed crude N-(1-(4-chlorobenzofuran-7-yl)-3,3-dimethylbutan-2-yl)formamide (20 g) and 300 mL of anhydrous DCM. Oxalyl chloride (10 g) was added dropwise at room temperature under a nitrogen atmosphere. Then the mixture was cooled to −10° C. Anhydrous FeCl₃ (13.9 g) was added portionwise. The reaction was slowly warmed to ambient temperature and stirred for 16 h. The reaction was quenched with 2N HCl. The organic layers was washed with brine solution, dried, filtered and concentrated. The residue was dissolved in concentrated H₂SO₄/MeOH (1/19, 200 mL) and stirred at 70° C. for 2 hours. The mixture was cooled and concentrated. The residue was basified with aq. NaHCO₃ and extracted with DCM. The combined organic layers were dried, filtered and concentrated to afford crude 8-(tert-butyl)-4-chloro-8,9-dihydrofuro[2,3-f]isoquinoline (13 g), which was used in next step without further purification.

Step 7: Into a 500 mL round bottom flask was added crude 8-(tert-butyl)-4-chloro-8,9-dihydrofuro[2,3-f]isoquinoline (13 g) and EtOH/H₂O mixture (3/1, 150 mL), then ethyl 2-(ethoxymethylene)-3-oxobutanoate (27.8 g) was added. The mixture was stirred at 100° C. for 22 h. The mixture was concentrated and purified by silica gel chromatography to afford ethyl 5-(tert-butyl)-12-chloro-9-oxo-4,9,10,10a-tetrahydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylate (5 g, 13% yield for 4 steps).

Step 8: Into a 250 mL round bottom flask was added ethyl 5-(tert-butyl)-12-chloro-9-oxo-4,9,10,10a-tetrahydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylate (5 g) and 50 mL of DME, then p-chloranil (6.1 g) was added. The mixture was heated to 70° C. and stirred for 1 hour. The mixture was directly concentrated and purified by silica gel chromatography to furnish ethyl 5-(tert-butyl)-12-chloro-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylate (2.6 g, 52% yield).

Step 9: Ethyl 5-(tert-butyl)-12-chloro-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylate (150 mg) was dissolved in H₂O/THF/MeOH (4/1/3, 15 mL), then NaOH (40 mg) was added. After stirring at room temperature for 2 h, the volatiles were removed. The aqueous layer was brought to pH=3 by addition of 5N HCl. The product was extracted with DCM, and concentrated. The residue was purified by SFC (Column: CHIRAL ART Cellulose-SB, mobile phase: 20% CO₂/MeOH to provide the product, (S)-5-(tert-butyl)-12-chloro-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid as a white solid (88 mg, 59% yield). ESI MS m/z=372.1 [M+H]*.

Example 3: (S)-5-(tert-butyl)-12-(furan-3-yl)-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

An oven-dried vial was charged with (S)-5-(tert-butyl)-12-chloro-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid (50 mg), 4,4,5,5-tetramethyl-2-(5-methylfuran-2-yl)-1,3,2-dioxaborolane (124 mg), Pd^(t)BuXPhos G3 (10 mg), and Cs₂CO₃ (194 mg). The vial was purged with nitrogen gas for 5 minutes, then DMF (3 mL) and water (0.8 mL) were added via syringe. The reaction mixture was heated under a nitrogen atmosphere for 80 minutes at 110° C. After cooling to room temperature, the reaction mixture was filtered and the product was purified by RPHPLC to provide the product as a white solid (4 mg). ESI MS m/z=404.1 [M+H]⁺.

Example 4: (S)-5-(tert-butyl)-12-(1-methyl-1H-pyrazol-5-yl)-9-oxo-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=418.1 [M+H]⁺.

Example 5: (S)-5-(tert-butyl)-12-(1-methyl-1H-pyrazol-4-yl)-9-oxo-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=418.1 [M+H]⁺.

Example 6: (S)-5-(tert-butyl)-9-oxo-12-(1H-pyrazol-5-yl)-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=404.1 [M+H]⁺.

Example 7: (S)-5-(tert-butyl)-9-oxo-12-(thiophen-3-yl)-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=420.1 [M+H]⁺.

Example 8: (S)-5-(tert-butyl)-12-(furan-2-yl)-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=404.1 [M+H]⁺.

Example 9: (S)-5-(tert-butyl)-9-oxo-12-(1H-pyrazol-4-yl)-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=404.1 [M+H]⁺.

Example 10: (S)-5-(tert-butyl)-9-oxo-12-(thiophen-2-yl)-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=420.1 [M+H]⁺.

Example 11: (S)-5-(tert-butyl)-12-(isothiazol-4-yl)-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=421.1 [M+H]⁺.

Example 12: (S)-5-(tert-butyl)-12-(oxazol-5-yl)-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=405.1 [M+H]⁺.

Example 13: (S)-5-(tert-butyl)-12-(furan-3-yl)-9-oxo-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=404.1 [M+H]⁺.

Example 14: (S)-5-(tert-butyl)-12-(1-methyl-1H-pyrazol-5-yl)-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=418.1 [M+H]⁺.

Example 15: (S)-5-(tert-butyl)-12-(1-methyl-1H-pyrazol-4-yl)-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=418.1 [M+H]⁺.

Example 16: (S)-5-(tert-butyl)-12-(1-methyl-1H-pyrazol-3-yl)-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=418.1 [M+H]⁺.

Example 17: (S)-5-(tert-butyl)-9-oxo-12-(thiazol-4-yl)-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=421.1 [M+H]⁺.

Example 18: (S)-5-(tert-butyl)-9-oxo-12-(pyrrolidin-1-yl)-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

An oven-dried vial was charged with (S)-5-(tert-butyl)-12-chloro-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid (50 mg), pyrrolidine (100 mg), Pd-RuPhos G3 (10 mg), and Cs₂CO₃ (194 mg). The vial was purged with nitrogen gas for 5 minutes, then 1,4-dioxane (5 mL) was added via syringe. The reaction mixture was heated under a nitrogen atmosphere for 2 h at 90° C. After cooling to room temperature, the reaction mixture was filtered and the product was purified by RPHPLC to provide the product as a yellow solid (15 mg). ESI MS m/z=407.1 [M+H]⁺.

Example 19: (S)-5-(tert-butyl)-12-(3,3-difluoropyrrolidin-1-yl)-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 18. ESI MS m/z=443.1 [M+H]⁺.

Example 20: (S)-5-(tert-butyl)-12-(3,3-difluoropyrrolidin-1-yl)-9-oxo-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 18. ESI MS m/z=443.1 [M+H]⁺.

Example 21: (S)-5-(tert-butyl)-9-oxo-12-(pyrrolidin-1-yl)-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 18. ESI MS m/z=406.1 [M+H]⁺.

Example 22: (S)-5-(tert-butyl)-12-(oxazol-5-yl)-9-oxo-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=405.1 [M+H]⁺.

Example 23: (S)-5-(tert-butyl)-12-(1-methyl-1H-pyrazol-3-yl)-9-oxo-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=418.1 [M+H]⁺.

Example 24: (S)-5-(tert-butyl)-9-oxo-12-(thiophen-2-yl)-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=420.1 [M+H]⁺.

Example 25: (S)-5-(tert-butyl)-9-oxo-12-(thiophen-3-yl)-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=420.1 [M+H]⁺.

Example 26: (S)-5-(tert-butyl)-12-(furan-2-yl)-9-oxo-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=404.1 [M+H]⁺.

Example 27: (S)-5-(tert-butyl)-9-oxo-12-(1H-pyrazol-4-yl)-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=404.1 [M+H]⁺.

Example 28: (S)-5-(tert-butyl)-9-oxo-12-(1H-pyrazol-5-yl)-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=404.1 [M+H]⁺.

Example 29: (S)-5-(tert-butyl)-12-(isothiazol-4-yl)-9-oxo-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=421.1 [M+H]⁺.

Example 30: (S)-5-(tert-butyl)-9-oxo-12-(thiazol-4-yl)-4,9-dihydro-5H-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

The title compound was prepared following similar procedure as Example 3. ESI MS m/z=421.1 [M+H]⁺.

Example 31: (S)-5-(tert-butyl)-9-oxo-4,9-dihydro-11-furo[3,2-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid

An oven-dried vial was charged with (S)-5-(tert-butyl)-12-chloro-9-oxo-4,9-dihydro-5H-furo[2,3-f]pyrido[2,1-a]isoquinoline-8-carboxylic acid (50 mg), Pd-XPhos G3 (10 mg), and Cs₂CO₃ (194 mg). The vial was purged with nitrogen gas for 5 minutes, then 1,4-dioxane (5 mL) was added via syringe. The reaction mixture was heated under a nitrogen atmosphere for 1 h at 100° C. After cooling to room temperature, the reaction mixture was filtered and the product was purified by RPHPLC to provide the product as a white solid (2 mg). ESI MS m/z=338.1 [M+H]⁺.

The following examples are prepared by using procedures similar to those described above.

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Biological Activity Methods:

2.2.15 cells are passaged upon attaining confluency in DMEM/F12 media in the presence of 10% FBS, Penn/Strep, and 250 ug/mL G418. Novel compounds are 5 fold serially diluted in DMSO and added to 96 well plates containing 35,000 cells/well at a 1:200 dilution so that the final concentration of DMSO is 0.5%. On day 5, post treatment cell lysates and supernatants are harvested for analysis.

Cells are lysed using Agilent Sidestep Lysis buffer, diluted 1:100 and quantified via quantitative real time PCR. Commercially available ELISA kits are used to quantitate the viral proteins HBsAg (Alpco) or HBeAg (US Biological) by following the manufacturer's recommended protocol after diluting samples to match the linear range of their respective assays. Irrespective of readout, compound concentrations that reduce viral product accumulation in the cell lysates or supernatants by 50% relative to no drug controls (EC₅₀) are reported; EC₅₀ ranges are as follows: A<0.1 μM; B 0.1-1 μM; C>1 μM.

Additionally, compound induced cellular toxicity is evaluated by exposing HepG2 cells seeded at 5,000 cells/well to serially diluted compound with a final DMSO concentration of 0.5% for three days. At day 3, post seeding cells are treated with ATPlite 1Step according to the manufacturer's instructions. Compound concentrations that reduce total ATP levels in wells by 50% relative to no drug controls (CC₅₀) are reported; CC₅₀ ranges are as follows: A>25 μM; B 10-25 M; CK<10 μM.

TABLE 2 Summary of Activities Example 2.2.15 cells HepG2 cells Example 2.2.15 cells HepG2 cells Number EC₅₀ (μM) CC₅₀ (μM) Number EC₅₀ (μM) CC₅₀ (μM) 1 A A 2 A A 3 A A 4 A 5 C A 6 A B 7 A B 8 A A 9 A A 10 A B 11 A A 12 A A 13 A B 14 A A 15 A A 16 A B 17 A A 18 A A 19 A B 20 B 21 B 22 B 23 C 24 C 25 C 26 C 27 C 28 C 29 C 30 C 31 C

While this invention has been particularly shown and described with references to preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention encompassed by the appended claims. 

1. A compound represented by Formula (I):

or a pharmaceutically acceptable salt thereof, wherein: R₁ is hydrogen, halo, or optionally substituted C₁-C₆ alkyl; R₂ is hydrogen, halo, CN, optionally substituted C₁-C₆ alkyl, or optionally substituted C₁-C₆ alkoxy; R₃ is hydrogen, halo, CN, optionally substituted C₁-C₆ alkyl, optionally substituted C₂-C₆ alkenyl; optionally substituted C₁-C₆ alkoxy, optionally substituted C₃-C₁₂ cycloalkyl, optionally substituted C₃-C₁₂ cycloalkenyl, optionally substituted 3- to 12-membered heterocyclic, optionally substituted aryl, or optionally substituted heteroaryl; A is optionally substituted 3- to 12-membered heterocyclic, optionally substituted aryl, or optionally substituted heteroaryl; R₄ is hydrogen, halo, optionally substituted C₁-C₆ alkyl, or optionally substituted C₁-C₆ alkoxy; R₅ is hydrogen, halo, optionally substituted C₁-C₆ alkyl, or optionally substituted C₁-C₆ alkoxy; R₆ is hydrogen or optionally substituted C₁-C₆ alkyl; R₇ is hydrogen, halo, optionally substituted C₁-C₆ alkyl, or optionally substituted C₁-C₆ alkoxy; R₈ is hydrogen or optionally substituted methyl; W is —COOR₁₁, —C(O)NHSO₂R₁₂, —C(O)NHSO₂N(R₁₂R₁₃), 5-tetrazolyl, or 1,2,4-oxadiazol-3-yl-5(4H)-one; R₁₁ is hydrogen or optionally substituted C₁-C₆ alkyl; and R₁₂ and R₁₃ are each independently selected from hydrogen and optionally substituted C₁-C₆ alkyl; alternatively, R₆ is taken together with R₅ and the carbon atoms to which they are attached to form a C₃-C₇ cycloalkyl ring or a 3- to 7-membered heterocyclic ring; or R₆ is taken together with R₅ and the carbon atoms to which they are attached to form a C₄-C₇ cycloalkenyl ring; wherein the cycloalkyl, cycloalkenyl or heterocyclic ring is optionally substituted with up to three groups selected from R₁₂, —OR₁₂, —N(R₁₂)(R₁₃), halo, CN, COOR₁₂, CON(R₁₂)(R₁₃), and oxo; or R₇ is taken together with R₆ and the carbon atom to which they are attached to form a C₃-C₇ cycloalkyl ring or a 3- to 7-membered heterocyclic ring; wherein the cycloalkyl or heterocyclic ring is optionally substituted with up to three groups selected from R₁₂, —OR₁₂, —N(R₁₂)(R₁₃)₂, halo, CN, COOR₁₂, CON(R₁₂)(R₁₃)₂, and oxo.
 2. A compound of claim 1, wherein A is optionally substituted 5- to 7-membered unsaturated heterocyclic, or optionally substituted 5- to 6-membered heteroaryl.
 3. A compound of claim 1, wherein A is selected from the groups set forth below:

wherein the two indicated valences are the points of attachment to either position of the phenyl ring in Formula (I) which is fused with the A ring.
 4. The compound of claim 1, represented by Formula (V), or a pharmaceutically acceptable salt thereof:

wherein one U is —O—, —S—, or —NR₂₂—, and the other Us are independently —NR₂₂— or —C(R₂₁)₂—; at least one pair of adjacent Us are taken together to form —C(R₂₁)═C(R₂₁)—, —C(R₂₁)═N—, or —N═N—; m is 0, 1, 2 or 3; R₁, R₃, R₆, and W are as defined in claim
 1. 5. The compound of claim 1, represented by Formulae (VII-1)˜(VII-4), or a pharmaceutically acceptable salt thereof:

wherein R₃ and R₆ are as defined in claim
 1. 6. The compound of claim 1, represented by Formulae (VIII-1)˜(VIII-4), or a pharmaceutically acceptable salt thereof:

wherein R₁, R₃ and R₆ are as defined in claim
 1. 7. The compound of claim 1, represented by Formulae (VI-1)˜(VI-2), or a pharmaceutically acceptable salt thereof:

wherein R₃, A, R₆, and W is as defined in claim
 1. 8. The compound of claim 1, selected from the compounds set forth below, or a pharmaceutically acceptable salt thereof: Compound Structure  1

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9. A pharmaceutical composition, comprising a compound according to claim 1 and a pharmaceutically acceptable carrier or excipient.
 10. A method of treating or preventing an HBV infection in a subject in need thereof, comprising administering to the subject a therapeutically effective amount of a compound or a combination of compounds according to claim
 1. 11. The method of claim 10, further comprising administering to the subject an additional therapeutic agent selected from the group consisting of a HBV polymerase inhibitor, interferon, viral entry inhibitor, viral maturation inhibitor, literature-described capsid assembly modulator, reverse transcriptase inhibitor, TLR-agonist, inducer of cellular viral RNA sensor, therapeutic vaccine, and agents of distinct or unknown mechanism, and a combination thereof.
 12. The method of claim 11, wherein the compound and the additional therapeutic agent are co-formulated.
 13. The method of claim 11, wherein the compound and the additional therapeutic agent are co-administered.
 14. The method of claim 11, wherein the additional therapeutic agent is administered at a lower dose or frequency compared to the dose or frequency of the additional therapeutic agent that is required to treat an HBV infection when administered alone.
 15. The method of claim 11, wherein the subject is refractory to at least one compound selected from the group consisting of a HBV polymerase inhibitor, interferon, viral entry inhibitor, viral maturation inhibitor, distinct capsid assembly modulator, inducer of cellular viral RNA sensor, therapeutic vaccine, antiviral compounds of distinct or unknown mechanism, and combination thereof.
 16. The method of claim 11, wherein the administering of the compound reduces viral load in the individual to a greater extent compared to the administering of a compound selected from the group consisting of a HBV polymerase inhibitor, interferon, viral entry inhibitor, viral maturation inhibitor, distinct capsid assembly modulator, inducer of cellular viral RNA sensor, therapeutic vaccine, antiviral compounds of distinct or unknown mechanism, and combination thereof.
 17. The method of claim 11, wherein the administering of the compound causes a lower incidence of viral mutation and/or viral resistance than the administering of a compound selected from the group consisting of a HBV polymerase inhibitor, interferon, viral entry inhibitor, viral maturation inhibitor, distinct capsid assembly modulator, inducer of cellular viral RNA sensor, therapeutic vaccine, antiviral compounds of distinct or unknown mechanism, and combination thereof. 